FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-10-5-1-3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-10-5-1-3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11496
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT286724.93910925539318No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTAA9978.672581767571328No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT8747.602644398051496No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCTG7656.654488517745302No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTAA3673.19241475295755No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGAT3422.9749478079331944No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT2712.3573416840640222No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGATC2652.3051496172581767No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATCT2602.2616562282533055No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT2141.8615170494084898No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGAT1971.7136395267919275No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTAA1791.5570633263743912No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1681.4613778705636742No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCGA1241.0786360473208072No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT1211.0525400139178844No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1110.9655532359081419No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCGA1100.9568545581071677No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT950.8263743910925538No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTA910.7915796798886569No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT870.75678496868476No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACCG860.7480862908837858No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAACC770.6697981906750173No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTA730.6350034794711203No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAACC670.5828114126652749No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTA580.5045233124565066No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTA470.4088378566457898No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAAC450.3914405010438413No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT450.3914405010438413No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTAA450.3914405010438413No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCGA400.3479471120389701No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAACCG400.3479471120389701No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTAACC380.33054975643702156No Hit
CTAGGTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTAACCG360.31315240083507306No Hit
CTAGGTAGAACAGGCTCCTCTAGCACAGGGGTATCTAATCCCAGTAACCG350.3044537230340988No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA340.29575504523312457No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT330.2870563674321503No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG330.2870563674321503No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCCC320.2783576896311761No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACCG300.2609603340292276No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTAA300.2609603340292276No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGAT280.2435629784272791No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACCGAT270.23486430062630478No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCGAT270.23486430062630478No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGGGTATCTAATCCCAGTAACCGA250.21746694502435632No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAAC250.21746694502435632No Hit
CTAGGTAGAACAGGCTCCTCTAGATGTGTATAAGAGACAGTGATTGACAC240.20876826722338201No Hit
CTAGGTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTAAC240.20876826722338201No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG220.19137091162143355No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGTGGGGTATCTAATCCCAGTAA210.18267223382045927No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG210.18267223382045927No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT210.18267223382045927No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG210.18267223382045927No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAACCG200.17397355601948505No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTGCCCGGGGTATCTAATCCCAGT200.17397355601948505No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT190.16527487821851078No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT190.16527487821851078No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGTAA180.15657620041753653No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT180.15657620041753653No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTAAC170.14787752261656228No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC170.14787752261656228No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGAT170.14787752261656228No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGAT160.13917884481558804No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA160.13917884481558804No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGCGGGGTATCTAATCCCAGTAACCG160.13917884481558804No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAACCG150.1304801670146138No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTAAC150.1304801670146138No Hit
CTAGGTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAATCC140.12178148921363954No Hit
CTAGGTAGAACAGGCTCCTCTAGGGCAGCGCAGGGGTATCTAATCCCAGT140.12178148921363954No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT140.12178148921363954No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCGA140.12178148921363954No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAGGTCCTT140.12178148921363954No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT130.11308281141266527No Hit
CTAGGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACCGA130.11308281141266527No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTAAC130.11308281141266527No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAATC130.11308281141266527No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT120.10438413361169101No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT120.10438413361169101No Hit
CTAGGTAGAACAGGCTCCTCTAGCCATAAAATCCGTGGTTTTGCTATCAA120.10438413361169101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGC108.3560945E-4295.08
CTAGGTA11300.0295.01
CACAGCG108.3560945E-4295.0295
GGTAGAA11350.0293.700444
TAGGTAG11350.0293.700442
AGGTAGA11400.0292.412263
AACAGGC11200.0292.36619
GAACAGG11350.0288.502238
AGAACAG11650.0282.339057
GTAGAAC11800.0281.255
TAGAACA11700.0281.132486
AGAACGG150.0028131178196.666667
AACGGCT150.0028131178196.666669
AGCGAGA150.0028131178196.66666295
CTAGGAA352.0690834E-4126.428581
TAGGAAG403.5203795E-4110.6252
GGAAGAA455.6239753E-498.3333364
AGGAAGA508.54904E-488.53
TCCTTAG452.5465852E-1159.00000445-49
CAGTTTT3050.059.00000485-89
Produced by FastQC (version 0.11.5)