FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-1-5-8-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-1-5-8-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11036
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT365433.10982239942008No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT126711.480608916274013No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG6946.2885103298296485No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG4403.9869517941283075No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG3393.0717651322943094No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3393.0717651322943094No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2762.5009061254077563No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG1921.7397607828923523No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1491.350126857557086No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1351.2232693004711852No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1311.1870242841609278No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT1121.0148604566872055No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT1050.9514316781442551No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1030.9333091699891265No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG1010.9151866618339978No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG770.6977165639724537No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA760.6886553098948894No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT720.6524102935846321No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT700.6342877854295035No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT660.598042769119246No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG660.598042769119246No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC640.5799202609641174No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT570.516491482421167No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC570.516491482421167No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT500.45306270387821673No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT490.44400144980065237No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT410.3715114171801377No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT390.3533889090250091No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTGTGT380.3443276549474447No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA340.3080826386371874No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTGTT320.2899601304820587No Hit
GAGACTTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG310.2808988764044944No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT280.25371511417180137No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT260.2355926060166727No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT260.2355926060166727No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT250.22653135193910837No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG250.22653135193910837No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC230.2084088437839797No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG230.2084088437839797No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT220.19934758970641536No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGTGT220.19934758970641536No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT220.19934758970641536No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT220.19934758970641536No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT210.19028633562885103No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT210.19028633562885103No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTG200.1812250815512867No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGTTG200.1812250815512867No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT200.1812250815512867No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT190.17216382747372236No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT190.17216382747372236No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT180.16310257339615802No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG170.1540413193185937No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC170.1540413193185937No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT160.14498006524102935No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA160.14498006524102935No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTAGATGTGTATAAGAGGGGTATCT160.14498006524102935No Hit
GAGACTTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTG160.14498006524102935No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT150.13591881116346502No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT150.13591881116346502No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT130.11779630300833635No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT130.11779630300833635No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA130.11779630300833635No Hit
GAGACTTAGAACAGGCTCCTCTTATAGGGTATCTAATCCCAGTGTGTTGC130.11779630300833635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA10700.0295.05
ACTTAGA10850.0295.04
GACTTAG10850.0295.03
GAGACTT10950.0295.01
CTTAGAC157.047409E-6294.999975
TTAGAAC10750.0293.627936
AGACTTA11000.0292.318182
GAACAGG10700.0292.242989
TAGAACA11000.0284.272747
AGAACAG11250.0277.955578
GACAGGC255.408946E-5177.09
AGACAGG255.408946E-5177.08
TTAGACA301.1186185E-4147.499986
TAGACAG352.0668717E-4126.4285667
CCGTGTC257.7359055E-659.000004215-219
GAGCGGT501.8189894E-1259.00000485-89
CAGTTTT4300.059.00000485-89
TATATTT650.059.00000495-99
TCTGGTG257.7359055E-659.00000475-79
TAAGCGT257.7359055E-659.00000455-59
Produced by FastQC (version 0.11.5)