FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11636895
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1724581.4819932636669835No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA1325651.1391784492340955No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1075810.9244820031460281No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC935540.8039429761976885No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA905710.7783089904996136No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG679560.5839702085478987No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC655250.5630797562408185No Hit
TCGGTCAAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT647190.5561535100213588No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT623680.5359505263216691No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG619440.5323069427024992No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT530370.45576590662715444No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC472840.40632832039818184No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG387570.33305276020794206No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG372450.32005960352826074No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC333970.2869923635127755No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA327190.28116606706514063No Hit
TCGGTCAAGTGTCAGCAGCCGCGTAGTCCTTCCTCTGTCTCTTATACACA302500.25994906716954996No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT246420.21175751779147273No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG215370.18507514246712717No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC202700.17418735839757943No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT186100.1599223847942256No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT177760.15275552456217917No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC173780.14933536824041121No Hit
TCGGTCAAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGAT165920.1425809891727991No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC158410.13612737762092036No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT157040.13495008763076405No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA156020.1340735651563411No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA154280.13257832093526667No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC138680.11917268309115103No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC134390.11548613268401924No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA133350.11459242349441151No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA132230.11362996744406476No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG131550.1130456191277828No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA126980.10911845470806429No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT123990.10654904078794215No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA122220.10502801649409056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCA1071650.0291.660581
ATCGGTT265900.0287.05261
CTGGATG493650.0284.573761
GGTACAC295300.0274.15841
GGTCAAG276400.0273.18463
TCAAGTG279200.0267.546485
GGATGAC447650.0264.36993
CGACTGG260600.0263.249764
GCGACTG260550.0262.06333
TCGGTTG85200.0257.597782
AGCGACT265950.0255.029852
GGTTACT218850.0249.164934
GATGACT479450.0248.027054
ATGACTG486900.0245.588675
TGGTGCC37050.0245.447056
AAGCGAC276400.0245.133821
GTACGTG107800.0244.747742
TGGTGTC56450.0241.512396
CGGTCAA1295450.0240.87152
GACACTG688550.0240.60996
Produced by FastQC (version 0.11.5)