FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-99-4-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-99-4-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22220
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG454220.44104410441044No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC257411.584158415841586No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG16277.322232223222322No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCGC10174.576957695769577No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG8863.9873987398739876No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTCG6572.9567956795679566No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAC6402.8802880288028803No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4702.115211521152115No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCCT4201.8901890189018902No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTCG3581.611161116111611No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3491.5706570657065706No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAC3251.4626462646264626No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3161.4221422142214222No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGCC2631.1836183618361835No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCGC2601.17011701170117No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACTT2371.0666066606660667No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTTCG1950.8775877587758776No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1920.8640864086408641No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1870.8415841584158417No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTCGC1780.801080108010801No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAACTT1630.7335733573357336No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACTTC1540.6930693069306931No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAACT1540.6930693069306931No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTCG1360.6120612061206121No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAACT1320.594059405940594No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACTTC1280.5760576057605761No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1250.5625562556255626No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1150.5175517551755175No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1070.4815481548154816No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA990.4455445544554455No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA970.4365436543654366No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAC960.43204320432043203No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAACT960.43204320432043203No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAACTTCG870.39153915391539157No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCGC850.38253825382538253No Hit
AAGCGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTAAC840.37803780378037805No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACTTC760.342034203420342No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG760.342034203420342No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA740.333033303330333No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG720.32403240324032406No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAACTT720.32403240324032406No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG720.32403240324032406No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAACT680.30603060306030605No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT670.30153015301530156No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAACT670.30153015301530156No Hit
AAGCGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAACTT620.27902790279027906No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG610.2745274527452745No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT610.2745274527452745No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCGC560.252025202520252No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG550.24752475247524752No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTCGC540.24302430243024303No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA530.23852385238523852No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAACT530.23852385238523852No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC530.23852385238523852No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG520.234023402340234No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG470.2115211521152115No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTCGC460.20702070207020704No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAAC450.20252025202520252No Hit
AAGCGACTGGGATTAGATACCCCAGCAGACCTAGAGGAGCCTGTTCTAAC440.19801980198019803No Hit
AAGCGACTGGGATTAGATACCCCCCGAGACATAGAGGAGCCTGTTCTAAC430.1935193519351935No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT410.1845184518451845No Hit
AAGCGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTAAC410.1845184518451845No Hit
AAGCGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAACT390.17551755175517553No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAACTTCG380.171017101710171No Hit
AAGCGACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTAA380.171017101710171No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG370.1665166516651665No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAACTTC360.16201620162016203No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA360.16201620162016203No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAACTT360.16201620162016203No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG340.15301530153015303No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGTGACCTAGAGGAGCC340.15301530153015303No Hit
AAGCGACTGGGATTAGATACCCCATGAGACCTAGAGGAGCCTGTTCTAAC330.1485148514851485No Hit
AAGCGACTGGGATTAGATACCCCCCGACCTAGAGGAGCCTGTTCTAACTT320.14401440144014402No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAACTTC310.13951395139513953No Hit
AAGCGACTGGGATTAGATACCCCTGATGCCGACGACTAGAGGAGCCTGTT310.13951395139513953No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTTCCTAGAGGAGCCTGTTCTA300.135013501350135No Hit
AAGCGACTGGGATTAGATACCCCACTGTTGGAGACTAGAGGAGCCTGTTC300.135013501350135No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCGACTAGAGGAGCCTGTTCTAA280.126012601260126No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA260.117011701170117No Hit
AAGCGACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTAAC250.11251125112511251No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT240.10801080108010801No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAAC230.10351035103510352No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAACT230.10351035103510352No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG230.10351035103510352No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT230.10351035103510352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG22100.0295.03
GACTGGG22100.0295.05
GGGATAG108.409296E-4295.09
TGGGATA108.409296E-4295.08
AGCGACT22100.0295.02
CCGACAA157.1452996E-6295.0295
CGACTGG22150.0294.334084
ACTGGGA22100.0294.332586
CTGGGAT22050.0294.331057
TGGGATT21850.0294.324958
GGGATTA21850.0293.64999
AAGCGAC22350.0292.360171
GCCCCAA600.059.000004115-119
CCCAGAC150.00437010359.00000420-24
AACTGTC150.00437010359.000004155-159
CGCCCAC150.00437010359.000004175-179
CCACGCT150.00437010359.00000420-24
GAAGCCT150.00437010359.000004135-139
CTGACGC150.00437010359.00000420-24
CCCACGC150.00437010359.00000420-24
Produced by FastQC (version 0.11.5)