FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-98-4-1-3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-98-4-1-3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22138
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC355016.035775589484143No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA254811.50962146535369No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACT19999.029722648839101No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA8703.929894299394706No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGC8263.7311410244827896No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT7803.5233535098021505No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCAC5932.6786520914265064No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAG5812.6244466528141657No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA4071.8384677929352247No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATA3571.6126117987171378No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGC3531.594543319179691No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3501.5809919595266058No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCA3421.5448550004517119No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAG3091.3957900442677749No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2941.328033246002349No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2891.3054476465805402No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2881.3009305266961784No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2671.2060710091245823No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2601.17445116993405No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAA2000.9034239768723461No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAT1850.8356671786069202No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1720.7769446201102178No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1350.6098111843888336No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1280.5781913451983016No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAG1220.5510886258921311No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC1180.5330201463546843No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1170.5285030264703225No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGC1060.4788147077423434No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT1050.47429758785798176No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1050.47429758785798176No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATA1040.46978046797362005No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA980.44267774866744963No Hit
ATCGGTTACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAT920.41557502936127927No Hit
ATCGGTTACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTATAAG910.41105790947691745No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT750.33878399132712983No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCA740.3342668714427681No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT740.3342668714427681No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT730.32974975155840636No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA720.3252326316740446No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT680.3071641521365977No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGC680.3071641521365977No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGC630.28457855271478905No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC620.28006143283042734No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGC610.2755443129460656No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT610.2755443129460656No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGC600.27102719306170386No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAA600.27102719306170386No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA590.26651007317734215No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT550.2484415936398952No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAG550.2484415936398952No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATAA540.24392447375553347No Hit
ATCGGTTACTGGGATTAGATACCCCCCGGCAATTACCGTGTAGTCGCCAA540.24392447375553347No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT530.2394073538711717No Hit
ATCGGTTACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTA510.23037311410244826No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT500.22585599421808653No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA500.22585599421808653No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT490.22133887433372482No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA480.21682175444936308No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT450.2032703947962779No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATA430.19423615502755445No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT410.18520191525883098No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAT410.18520191525883098No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATAA410.18520191525883098No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAT400.18068479537446924No Hit
ATCGGTTACTGGGATTAGATACCCCACGAAACCTAGAGGAGCCTGTTCTA400.18068479537446924No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT390.1761676754901075No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC370.16713343572138406No Hit
ATCGGTTACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTATAAG360.1626163158370223No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATAAG350.1580991959526606No Hit
ATCGGTTACTGGGATTAGATACCCCAAGCAACCTAGAGGAGCCTGTTCTA350.1580991959526606No Hit
ATCGGTTACTGGGATTAGATACCCCGACGACCTCCCAGGAACCTCAGAGA350.1580991959526606No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG340.15358207606829885No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGCA330.14906495618393711No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT330.14906495618393711No Hit
ATCGGTTACTGGGATTAGATACCCCACATGATGGTGATTTTATAACAGCA330.14906495618393711No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT280.12647935676212846No Hit
ATCGGTTACTGGGATTAGATACCCCAGGCCCACGAGACCTAGAGGAGCCT280.12647935676212846No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAA270.12196223687776674No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT260.11744511699340501No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC260.11744511699340501No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA260.11744511699340501No Hit
ATCGGTTACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATAAG260.11744511699340501No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA260.11744511699340501No Hit
ATCGGTTACTGGGATTAGATACCCCACTTGGGAGATCAATGGGGAAAGAC240.10841087722468154No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCT240.10841087722468154No Hit
ATCGGTTACTGGGATTAGATACCCCACCCGACCTAGAGGAGCCTGTTCTA230.10389375734031982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.409039E-4295.09
TTACTGG21850.0295.06
CTGGGAT21750.0295.09
ATCGGTT22000.0295.01
ATCGGTG108.409039E-4295.01
CTGGATT108.409039E-4295.09
ACTGGAT108.409039E-4295.08
TCGGTTA21900.0294.999972
GGTTACT21900.0294.326484
CGGTTAC21900.0294.326483
GTTACTG21900.0294.326485
TACTGGG21800.0294.32347
ACTGGGA21750.0294.321848
TACTGGA150.0028308963196.666677
ACTGGGT150.0028308963196.666678
ATCTCCA150.004370050559.000004170-174
TTACCGT150.004370050559.00000430-34
GATACGA150.004370050559.000004285-289
GGCAATT150.004370050559.00000425-29
GTCGCCA150.004370050559.00000440-44
Produced by FastQC (version 0.11.5)