FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-97-4-1-3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-97-4-1-3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC358316.257543445709878No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA299913.607695448976814No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA20049.092971550433322No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTG9964.519261309496801No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT6703.0400653387177274No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA5592.536412722900313No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5332.4184400381142517No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAG4902.2233313671219204No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTG3981.8058895594173965No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATT3931.7832025046508462No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT3861.751440627977676No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3641.651617587004855No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3531.6017060665184446No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC3011.3657606969463225No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2781.2614002450201915No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG2491.1298153273742002No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2331.0572167521212397No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGA2120.9619311221017288No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG2010.9120196016153184No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1870.8484958482689777No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTG1840.8348836154090477No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG1620.7350605744362266No Hit
ATCGGTTACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC1560.7078361087163665No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1390.6307001225100958No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1370.6216253006034757No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG1320.5989382458369255No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGA1270.5762511910703753No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATT1240.5626389582104452No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT1220.5535641363038251No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA1160.5263396705839648No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG1110.5036526158174146No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGA990.4492036843776941No Hit
ATCGGTTACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTA980.4446662734243841No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT980.4446662734243841No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTG910.41290439675121376No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG880.3992921638912837No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGAT870.39475475293797363No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGA860.39021734198466357No Hit
ATCGGTTACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT850.3856799310313535No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTG800.3629928762648033No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC790.35845546531149325No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA780.35391805435818324No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT750.3403058214982531No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA750.3403058214982531No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT740.33576841054494305No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT710.3221561776850129No Hit
ATCGGTTACTGGGATTAGATACCCCAGGGAGATCGAGGGCCTAGAGGAGC700.3176187667317029No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT680.3085439448250828No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC610.27678206815191253No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTG580.2631698352919824No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTG570.25863242433867234No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATT540.24502019147874224No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG500.22687054766550208No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT480.217795725758882No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA470.21325831480557195No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGT470.21325831480557195No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT450.20418349289895188No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGATT450.20418349289895188No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT450.20418349289895188No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT420.19057126003902172No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGCCGCACTAGAGGAGCCTG380.17242161622578156No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG370.16788420527247153No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT340.1542719724125414No Hit
ATCGGTTACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAGA330.14973456145923136No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCATAGAGGAGCCTGTTCTAG330.14973456145923136No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTG310.1406597395526113No Hit
ATCGGTTACTGGGATTAGATACCCCATGAGAGAGCTAGAGGAGCCTGTTC310.1406597395526113No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGATACCTAGAGGAGCCT300.13612232859930123No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGAT300.13612232859930123No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA290.1315849176459912No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAT290.1315849176459912No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC290.1315849176459912No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT270.12251009573937112No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAAGAGGAGCCTGTTCTA260.11797268478606109No Hit
ATCGGTTACTGGGATTAGATACCCCACTGTTGGAGACTAGAGGAGCCTGT260.11797268478606109No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA260.11797268478606109No Hit
ATCGGTTACTGGGATTAGATACCCCACATCTAGAGGAGCCTGTTCTAGAT260.11797268478606109No Hit
ATCGGTTACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG230.10436045192613096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT21650.0295.09
TCGGTTA21900.0295.02
ATCGGTT21950.0295.01
TACTGGG21700.0295.07
CGGTTAC21850.0295.03
TTACTGG21750.0294.999976
GGTTACG108.408782E-4294.999974
CGGGATT108.408782E-4294.999979
ACGGGAT108.408782E-4294.999978
GTTACGG108.408782E-4294.999975
TTACGGG108.408782E-4294.999976
GGTTACT21800.0294.323364
GTTACTG21800.0294.323365
ACTGGGA21700.0294.320288
TACGGGA150.0028308097196.666667
TAAGCCA200.0066931555147.49998295
CATCAGA600.001785303773.74999295
GCCCCAA452.5465852E-1159.000004120-124
AACGCTT501.8189894E-1259.00000490-94
TTACTTT452.5465852E-1159.00000450-54
Produced by FastQC (version 0.11.5)