FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-96-5-1-3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-96-5-1-3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21567
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC680331.54356192330876No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA15847.344554179997218No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT15447.159085640098298No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATT10364.803635183382019No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT6523.0231372003523904No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5882.726387536514119No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGG5152.3879074511985907No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3781.7526777020447908No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAG3361.557935735150925No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGAT3181.474474892196411No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3121.4466546112115732No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3081.4281077572216814No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGG2391.108174525896045No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGA2291.061807390921315No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2140.9922566884592201No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2030.9412528399870171No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1540.7140538786108407No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1440.6676867436361107No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1390.6445031761487457No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1390.6445031761487457No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGG1310.6074094681689618No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAT1160.537858765706867No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1140.528585338711921No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1100.510038484722029No Hit
ATCGGTTACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAA1100.510038484722029No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1090.505401771224556No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT1050.4868549172346641No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1050.4868549172346641No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1000.4636713497472991No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT960.44512449575740715No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT840.3894839337877313No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT820.3802105067927853No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAATAGG800.3709370797978393No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAG790.3663003663003663No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT790.3663003663003663No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAATAG780.3616636528028933No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT690.3199332313256364No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT690.3199332313256364No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA600.2782028098483795No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAATA580.2689293828534335No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAATA580.2689293828534335No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA570.2642926693559605No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC530.24574581536606854No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA490.22719896137617657No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG490.22719896137617657No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGA490.22719896137617657No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAA470.2179255343812306No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAT460.2132888208837576No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCCAGAGGAGCCTGTTCTA450.2086521073862846No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAG450.2086521073862846No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAATA450.2086521073862846No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAATA420.19474196689386564No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA410.19010525339639264No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT400.18546853989891965No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAA390.18083182640144665No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAATAGG390.18083182640144665No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA390.18083182640144665No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG370.17155839940650067No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT360.16692168590902767No Hit
ATCGGTTACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTAAT360.16692168590902767No Hit
ATCGGTTACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAAT360.16692168590902767No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT350.16228497241155468No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA350.16228497241155468No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT310.14373811842166273No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAATAGG300.13910140492418974No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA300.13910140492418974No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC270.12519126443177075No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCGACCTAGAGGAGCCTGTTCT270.12519126443177075No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGG260.12055455093429776No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA260.12055455093429776No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAATA250.11591783743682478No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT250.11591783743682478No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA250.11591783743682478No Hit
ATCGGTTACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT240.11128112393935179No Hit
ATCGGTTACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTT240.11128112393935179No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAATA240.11128112393935179No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT240.11128112393935179No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCC240.11128112393935179No Hit
ATCGGTTACTGGGATTAGATACCCCAAAAGAAACGCAGAAATGATTGTCA230.1066444104418788No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAT230.1066444104418788No Hit
ATCGGTTACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.1020076969444058No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT220.1020076969444058No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA220.1020076969444058No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC220.1020076969444058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT21050.0295.000034
TTACTGG21000.0295.06
ATCGGTT21300.0295.01
CGGTTAC21150.0295.03
TGACTGG108.407587E-4294.999976
TACTGGA108.407587E-4294.999977
GTGACTG108.407587E-4294.999975
TCGGTTA21300.0294.30752
TACTGGG20950.0294.295937
GTTACTG21150.0293.60525
ACTGGGA21050.0293.59868
CTGGGAT21000.0293.595259
GGTGACT150.0028304083196.666664
CGGTGAC150.0028304083196.666663
GACTGGG150.0028304083196.666667
TCGGTGA200.0066922093147.499982
ATCGGTG200.0066922093147.499981
CTGGATT200.0066922093147.499989
ACTGGAT200.0066922093147.499988
AACCTTG1550.059.00000440-44
Produced by FastQC (version 0.11.5)