FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-95-5-2-6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-95-5-2-6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21336
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA674031.589801274840646No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA15997.494375703037121No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA12285.755530558680165No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTG8924.1807274090738655No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA6983.2714660667416573No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6813.1917885264341956No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTC4382.0528683914510686No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT4272.0013123359580054No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA3351.5701162354705662No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3291.541994750656168No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2781.3029621297337832No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2561.1998500187476566No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTC2491.1670416197975253No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCT2311.0826771653543308No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2211.0358080239970004No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCT2161.0123734533183353No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA2100.984251968503937No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1890.8858267716535433No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCT1790.8389576302962131No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1730.8108361454818148No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1720.8061492313460819No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCC1700.7967754030746156No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1430.6702287214098238No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1180.553055868016498No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA1120.5249343832020997No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1080.5061867266591676No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGT1000.46869141357330335No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1000.46869141357330335No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA990.4640044994375703No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA990.4640044994375703No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAG940.44056992875890516No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCT920.43119610048743906No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCTC720.3374578177727784No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC710.33277090363704537No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTC710.33277090363704537No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAG650.3046494188226472No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA630.2952755905511811No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT610.28590176227971503No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA580.2718410198725159No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCTC580.2718410198725159No Hit
GCCACAACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC580.2718410198725159No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA560.26246719160104987No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTC530.24840644919385074No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAA530.24840644919385074No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAG520.24371953505811775No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAGT480.22497187851518563No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCT470.22028496437945258No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTC470.22028496437945258No Hit
GCCACAACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAAG470.22028496437945258No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAAGT460.21559805024371953No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAAGTCT430.20153730783652046No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCTC400.18747656542932134No Hit
GCCACAACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTCTA390.18278965129358832No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT380.17810273715785527No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAA350.16404199475065617No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC350.16404199475065617No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG350.16404199475065617No Hit
GCCACAACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAAGT330.1546681664791901No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA320.14998125234345708No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGTC320.14998125234345708No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAG310.14529433820772406No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTGACTAGAGGAGCCTGTTCT310.14529433820772406No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT310.14529433820772406No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAAG290.13592050993625795No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCTC290.13592050993625795No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA290.13592050993625795No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAAG280.13123359580052493No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCT270.1265466816647919No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAG260.12185976752905887No Hit
GCCACAACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAAGT260.12185976752905887No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT240.11248593925759282No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC240.11248593925759282No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAA240.11248593925759282No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAA230.10779902512185977No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG220.10311211098612673No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT220.10311211098612673No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA220.10311211098612673No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA220.10311211098612673No Hit
GCCACAACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAAGTC220.10311211098612673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG21050.0295.06
CACTGGG108.406898E-4295.05
TGGGATG108.406898E-4295.09
TGGGATA108.406898E-4295.09
ACTGGGG108.406898E-4295.07
CAACTGG21150.0294.302585
CTGGGAT21050.0293.598578
ACTGGGA21050.0293.598577
TGGGATT20850.0293.585149
GCCACAA21350.0292.92741
CCACAAC22250.0281.078642
CACAACT22450.0277.91763
ACAACTG22500.0277.300024
ACACTGG150.0028301778196.666674
GCCACAC200.006691664147.51
AGCCCCC150.00436961759.000004175-179
AGCCCCA150.00436961759.000004175-179
CTTCACA150.00436961759.000004120-124
CTTCAAC452.5465852E-1159.00000440-44
ACGCCTT150.00436961759.000004190-194
Produced by FastQC (version 0.11.5)