FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-94-5-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-94-5-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21105
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC642830.457237621416727No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT12335.842217484008529No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA12235.794835347074153No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGC11235.321013977730395No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6983.307273158019427No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGT5982.8334517886756694No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5482.5965411040037902No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT4892.3169864960909736No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGT3251.5399194503672116No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3251.5399194503672116No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACG2921.3835583984837716No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACG2841.345652688936271No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2621.2414119876806444No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2511.189291637052831No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2151.0187159440890783No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1820.8623548922056384No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1820.8623548922056384No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1770.8386638237384506No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGG1750.8291873963515755No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1690.80075811419095No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1630.7723288320303245No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1400.6633499170812603No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACG1340.6349206349206349No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1170.5543710021321961No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1090.5164652925846956No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1080.511727078891258No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTG1010.47855958303719504No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1010.47855958303719504No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAG960.4548685145700071No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG910.4311774461028192No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGAC890.4217010187159441No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGT880.41696280502250654No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGA860.4074863776356314No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT860.4074863776356314No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACG820.3885335228618811No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAC790.37431888178156836No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT750.35536602700781805No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA730.3458895996209429No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTA730.3458895996209429No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG700.33167495854063017No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT700.33167495854063017No Hit
CTGGATGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGAC610.289031035299692No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA600.28429282160625446No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG580.2748163942193793No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA560.26533996683250416No Hit
CTGGATGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAGA530.25112532575219143No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT520.24638711205875385No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT510.24164889836531628No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGT510.24164889836531628No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGCGCAGCTGTTGGAGACT470.222696043591566No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG470.222696043591566No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG460.2179578298981284No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC440.20848140251125327No Hit
CTGGATGACTGGGATTAGATACCCCCGTGATGCTAGAGGAGCCTGTTCTA430.2037431888178157No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCTAGAGGAGCCTGTTCTAGA400.18952854773750297No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT350.16583747927031509No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG350.16583747927031509No Hit
CTGGATGACTGGGATTAGATACCCCTAGGATTGTTGGCAAGCTAGAGGAG340.1610992655768775No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACG330.15636105188343996No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGT330.15636105188343996No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC330.15636105188343996No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGAC330.15636105188343996No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAG330.15636105188343996No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAG320.15162283819000236No Hit
CTGGATGACTGGGATTAGATACCCCGGATAGTCCCTAGAGGAGCCTGTTC290.13740819710968966No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCCAGAGGAGCCTGTTCTA280.13266998341625208No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA280.13266998341625208No Hit
CTGGATGACTGGGATTAGATACCCCGTTGGCCGAGATGTCTAGAGGAGCC260.12319355602937693No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG260.12319355602937693No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC260.12319355602937693No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT260.12319355602937693No Hit
CTGGATGACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTA260.12319355602937693No Hit
CTGGATGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG250.11845534233593934No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG250.11845534233593934No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT240.11371712864250177No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT240.11371712864250177No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA240.11371712864250177No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT230.1089789149490642No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGT220.10424070125562664No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGAC220.10424070125562664No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG220.10424070125562664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC20800.0295.03
CTGAGAT157.1402683E-6295.09
CTGGGTT108.406339E-4295.09
TGGAGAC108.406339E-4295.02
CTGGATT206.0430466E-8295.09
CTGGATG20900.0295.01
CTGGAGA108.406339E-4295.01
TGGATGA20850.0295.02
ACTGGAT206.0430466E-8295.08
TGACTGG20600.0294.2846
GATGACT20850.0293.585144
ATGACTG20800.0293.581735
GACTGGG20550.0293.564457
CTGGGAT20450.0293.557439
ACTGGGA20450.0293.557438
GACTGGA251.8390347E-7236.07
AGACTGG150.0028299894196.666675
ACTGGGT150.0028299894196.666678
GGAGACT200.0066912207147.53
TGACTGA301.1332778E-4147.56
Produced by FastQC (version 0.11.5)