FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-93-4-8-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-93-4-8-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21609
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA300013.883104262113008No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC289513.397195612939052No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG21079.750566893424036No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC8804.072377250219816No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC7803.609607108149382No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA5982.767365449581193No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC5962.7581100467397843No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5812.688694525429219No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT5172.392521634504142No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT4512.0870933407376557No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3611.6706002128742652No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT3361.5549076773566568No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3081.4253320375769354No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCC2981.3790550233698922No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2881.332778009162849No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA2691.2448516821694664No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT2611.2078300708038316No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC2311.0689990281827018No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT2231.031977416817067No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2060.9533064926650933No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2030.9394233884029802No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC1710.7913369429404414No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1230.5692072747466334No Hit
CAACACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAC1150.5321856633809987No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1140.5275579619602944No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTC990.4581424406497293No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA990.4581424406497293No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTC960.4442593363876163No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTC950.43963163496691193No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA920.42574853070479896No Hit
CAACACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCACTCC910.4211208292840946No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACC890.4118654264426859No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTC860.3979823221805729No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACC840.3887269193391642No Hit
CAACACACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTCTA820.37947151649775557No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCAC820.37947151649775557No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACCA790.3655884122356426No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACC730.3378222037114165No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA710.3285668008700079No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC700.32393909944930355No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAC690.31931139802859915No Hit
CAACACACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTACCAC620.28691748808366885No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCAC610.2822897866629645No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA610.2822897866629645No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTC600.27766208524226016No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT600.27766208524226016No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG600.27766208524226016No Hit
CAACACACTGGGATTAGATACCCCCATAATATTAGCTAGAGGAGCCTGTT520.2406404738766255No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCAC490.22675736961451248No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA470.2175019667731038No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA470.2175019667731038No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC460.21287426535239948No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCA460.21287426535239948No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCAC440.2036188625109908No Hit
CAACACACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGT400.18510805682817347No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA390.1804803554074691No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACCA380.17585265398676478No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACC380.17585265398676478No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACC380.17585265398676478No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA350.16196954972465177No Hit
CAACACACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTAC340.15734184830394743No Hit
CAACACACTGGGATTAGATACCCGTGTAGTCCCTAGAGGAGCCTGTTCTA330.15271414688324308No Hit
CAACACACTGGGATTAGATACCCCACGAGACATAGAGGAGCCTGTTCTAC330.15271414688324308No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCACT320.14808644546253877No Hit
CAACACACTGGGATTAGATACCCCTGCAGTCCTAGAGGAGCCTGTTCTAC310.14345874404183442No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT300.13883104262113008No Hit
CAACACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACCAC300.13883104262113008No Hit
CAACACACTGGGATTAGATACCCCCGGCCACCTAGAGGAGCCTGTTCTAC290.13420334120042574No Hit
CAACACACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTACC290.13420334120042574No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA290.13420334120042574No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT290.13420334120042574No Hit
CAACACACTGGGATTAGATACCCCGAGTCTAGAGGAGCCTGTTCTACCAC280.12957563977972142No Hit
CAACACACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTACCACTC280.12957563977972142No Hit
CAACACACTGGGATTAGATACCCCTTATACACATCTGACGCTAGAGGAGC260.12032023693831274No Hit
CAACACACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA260.12032023693831274No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACC250.11569253551760841No Hit
CAACACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTACCACT240.11106483409690407No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTACCA240.11106483409690407No Hit
CAACACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCAC240.11106483409690407No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCC240.11106483409690407No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA220.1018094312554954No Hit
CAACACACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC220.1018094312554954No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC220.1018094312554954No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTC220.1018094312554954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG157.1426184E-6295.05
CAACTGG157.1426184E-6295.04
CACTGGG21300.0295.06
CTGGATT157.1426184E-6295.08
TGGATTA157.1426184E-6295.09
TGGGATA157.1426184E-6295.09
ACTGGAT157.1426184E-6295.07
ACACTGG21600.0292.95145
CTGGGAT21400.0292.932228
ACTGGGA21400.0292.932227
TGGGATT21300.0292.230049
CACACTG22050.0286.972784
ACACACT23500.0269.265963
AACACAC27200.0232.637862
CAACACA27900.0226.801071
CACTGGA301.1336489E-4147.56
CTTAGCC4050.059.00000430-34
CCCAAGG303.3140896E-759.00000420-24
GGCCTAG303.3140896E-759.00000435-39
ACCCCCC150.004369783759.00000420-24
Produced by FastQC (version 0.11.5)