FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-92-5-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-92-5-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21074
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA315614.975799563443104No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG259212.299515991268862No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC15737.464173863528519No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG7893.743949890860776No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG7883.7392047072221692No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA7633.6205751162569992No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA6282.9799753250450793No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA4392.0831356173483915No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3901.8506216190566576No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3901.8506216190566576No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG3671.7414823953687006No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2861.357122520641549No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG2791.3239062351713011No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGA2501.1862959096517034No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCT2311.096137420518174No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA2221.0534307677707129No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2201.0439404004934991No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGC2171.0297048495776788No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC2040.96801746227579No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGC1990.944291544082756No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1990.944291544082756No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG1830.8683686058650469No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGA1460.6927968112365949No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA1430.6785612603207745No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGGACCTAGAGGAGCC1310.6216190566574926No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1280.6073835057416722No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1190.5646768529942109No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGC1110.5267153838853564No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1090.5172250166081427No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG1090.5172250166081427No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGAG1060.5029894656923223No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1000.4745183638606814No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA990.4697731802220746No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT960.45553762930625413No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT950.45079244566764737No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGC930.4413020783904337No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTG910.43181171111322003No Hit
GCCACAACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAGC890.42232134383600645No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT830.3938502420043656No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGC830.3938502420043656No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTAGC780.3701243238113315No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAG760.3606339565341179No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT750.355888772895511No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG750.355888772895511No Hit
GCCACAACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTAGCT740.35114358925690425No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC730.34639840561829743No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG710.3369080383410838No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCT700.332162854702477No Hit
GCCACAACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAG680.3226724874252634No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA670.31792730378665657No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA650.3084369365094429No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA620.2942013855936225No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGA620.2942013855936225No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA590.27996583467780206No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA570.27047546740058837No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCTG560.2657302837619816No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAG540.25623991648476796No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGC510.2420043655689475No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTG480.22776881465312707No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGC470.22302363101452025No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA470.22302363101452025No Hit
GCCACAACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT460.21827844737591345No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT460.21827844737591345No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTG450.21353326373730663No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCT450.21353326373730663No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA440.20878808009869984No Hit
GCCACAACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGCTG440.20878808009869984No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC420.19929771282148617No Hit
GCCACAACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGCT390.18506216190566574No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAG370.17557179462845213No Hit
GCCACAACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGCTG370.17557179462845213No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGA360.1708266109898453No Hit
GCCACAACTGGGATTAGATACCCCGGTCCTAGAGGAGCCTGTTCTAGCTG360.1708266109898453No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGCTGA350.1660814273512385No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTGA350.1660814273512385No Hit
GCCACAACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCT330.15659106007402485No Hit
GCCACAACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTA330.15659106007402485No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT310.14710069279681126No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAG310.14710069279681126No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTAG290.13761032551959762No Hit
GCCACAACTGGGATTAGATACCCCCTCCGCGTGCTGCAGATCTAGAGGAG290.13761032551959762No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA280.1328651418809908No Hit
GCCACAACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGC280.1328651418809908No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA270.12811995824238398No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA260.12337477460377716No Hit
GCCACAACTGGGATTAGATACCCCTCTCCGAGCCCACGAGACCTAGAGGA250.11862959096517035No Hit
GCCACAACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA250.11862959096517035No Hit
GCCACAACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGC250.11862959096517035No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA230.10913922368795673No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGCTGAGA230.10913922368795673No Hit
GCCACAACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAGC220.10439404004934992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTGG20800.0295.05
TGTTAAA108.406197E-4295.0295
ACGGGAT108.406197E-4295.07
TGGGATT20500.0295.09
AACGGGA108.406197E-4295.06
CAACGGG108.406197E-4295.05
CTGGGAT20750.0294.289158
ACTGGGA20750.0294.289157
AACTGGG20850.0293.585146
GCCACAA21450.0289.49881
CCACAAC21900.0283.550232
CACAACT22000.0280.253
ACAACTG21950.0280.21644
TGGGATA202.25055E-5221.259
CGGGATT150.0028299422196.666668
TAGTCCC900.059.00000425-29
TCCGCCA1550.059.00000465-69
ATCCGCC1550.059.00000465-69
CCCCACC452.5465852E-1159.00000420-24
CAGCCCT201.839735E-459.030-34
Produced by FastQC (version 0.11.5)