FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-91-5-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-91-5-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20788
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA583528.06907831441216No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA13286.388300942851645No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC13146.320954396767366No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG11405.4839330382913225No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA7353.535693669424668No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC6283.0209736386376753No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA5372.5832210890898595No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT3841.8472195497402348No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT2941.414277467769867No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC2881.385414662305176No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2851.3709832595728306No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT2701.2988262459111026No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT2641.2699634404464115No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2611.2555320377140657No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2211.0631133346161246No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC2171.0438714643063305No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC2131.0246295939965364No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1890.9091783721377718No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACC1780.856263228785838No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC1490.7167596690398307No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1410.6782759284202424No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCC1380.6638445256878969No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1350.6494131229555513No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1230.591687512026169No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACC1160.5580142389840292No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1140.5483933038291322No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1030.49547816047719834No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1010.4858572253223013No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA990.47623629016740426No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA890.428131614392919No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACT880.4233211468154705No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT880.4233211468154705No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC850.40888974408312484No Hit
GCCACAACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAG830.3992688089282278No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC820.3944583413507793No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA770.37040600346353664No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA760.3655955358860881No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA740.355974600731191No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT710.34154319799884547No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACC660.3174908601116029No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA660.3174908601116029No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT610.29343852222436023No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC600.28862805464691166No Hit
GCCACAACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTACCAC580.2790071194920146No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT560.26938618433711753No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAC520.25014431402732346No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTAC500.2405233788724264No Hit
GCCACAACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTACCA500.2405233788724264No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCT500.2405233788724264No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA490.23571291129497787No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCAC480.23090244371752935No Hit
GCCACAACTGGGATTAGATACCCCTATAGTCCTAGAGGAGCCTGTTCTAC470.2260919761400808No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCAC460.2212815085626323No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA460.2212815085626323No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCAC450.21647104098518377No Hit
GCCACAACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTACCA440.21166057340773525No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACC430.20685010583028673No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTC420.20203963825283816No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTC420.20203963825283816No Hit
GCCACAACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG390.18760823552049258No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCAC380.18279776794304406No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAC380.18279776794304406No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA370.1779873003655955No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACC370.1779873003655955No Hit
GCCACAACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGT360.173176832788147No Hit
GCCACAACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA350.16836636521069848No Hit
GCCACAACTGGGATTAGATACCCCCAGTAGAGGAGCCTGTTCTACCACTC350.16836636521069848No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTC340.16355589763324996No Hit
GCCACAACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT330.15874543005580144No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT320.1539349624783529No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT320.1539349624783529No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC310.14912449490090438No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTA270.12988262459111025No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACCAC270.12988262459111025No Hit
GCCACAACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA270.12988262459111025No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA260.12507215701366173No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA260.12507215701366173No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG260.12507215701366173No Hit
GCCACAACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAC250.1202616894362132No Hit
GCCACAACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCAC230.11064075428131614No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA230.11064075428131614No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG220.10583028670386763No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCT220.10583028670386763No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA220.10583028670386763No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC220.10583028670386763No Hit
GCCACAACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACCAC220.10583028670386763No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG210.10101981912641908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG20400.0295.06
CTGGGAT20400.0295.08
ACTGGGA20350.0295.07
AACTTGG108.4053323E-4294.999976
CAACTGG20550.0294.282265
TGGGATT20450.0294.278729
GCCACAA20850.0292.87771
CCACAAC21350.0285.327882
CACAACT21500.0282.651153
ACAACTG21800.0277.408264
CTACAAA700.003287143363.214283295
CAGTTAA6900.059.00000845-49
AACTGCT6150.059.00000460-64
CTTAGCC8100.059.00000430-34
GCCAGAA6950.059.00000470-74
TATCCGC3100.059.00000465-69
ATGCCTA2150.059.00000425-29
GCGCCAG2150.059.00000485-89
TCAGACC2150.059.000004120-124
CTAGCCG2150.059.00000430-34
Produced by FastQC (version 0.11.5)