FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-90-4-1-3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-90-4-1-3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC357317.01347554878339No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA312914.899290509975716No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCC16818.004380743774107No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTC7653.6426836817294417No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT7243.447454883100805No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5682.7046331127089185No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTT5582.657016332555593No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCG4101.9522879862863673No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGC4061.9332412742250369No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTC3371.6046854911670874No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3201.523736964906433No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT3011.4332650826151137No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2761.3142231322317985No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2631.2523213180324746No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTT2411.1475644016951574No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT2331.1094709775724965No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2201.0475691633731727No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2000.9523356030665207No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA1980.9428122470358555No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1970.9380505690205229No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1870.8904337888671967No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACT1410.6713966001618971No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAAC1380.6571115661158993No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1320.6285414980239036No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1290.6142564639779058No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTT1210.576163039855245No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTT990.47140612351792777No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAC980.46664444550259515No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAA980.46664444550259515No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT970.46188276748726254No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTC970.46188276748726254No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT960.45712108947192986No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAC930.4428360554259321No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC930.4428360554259321No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA860.4095043093186038No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA850.40474263130327126No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA820.39045759725727347No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC800.38093424122660824No Hit
ATCGGTTACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTA700.33331746107328225No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTC680.323794105042617No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG670.3190324270272844No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTC660.3142707490119518No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC650.30950907099661923No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT610.2904623589352888No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACT580.276177324889291No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC580.276177324889291No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTC570.27141564687395836No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT560.2666539688586258No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT540.25713061282796057No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAACT520.24760725679729537No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT510.24284557878196275No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA500.23808390076663016No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT500.23808390076663016No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAACTT500.23808390076663016No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA490.23332222275129758No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAACT460.21903718870529976No Hit
ATCGGTTACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTG440.20951383267463455No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACT430.2047521546593019No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT430.2047521546593019No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT420.19999047664396932No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT410.19522879862863673No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAACTTC400.19046712061330412No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA370.17618208656730633No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACT370.17618208656730633No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT360.1714204085519737No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTC360.1714204085519737No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTC350.16665873053664113No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT340.1618970525213085No Hit
ATCGGTTACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTA320.1523736964906433No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAA300.1428503404599781No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGTCTAGAGGAGCCTGTT300.1428503404599781No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT300.1428503404599781No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT290.1380886624446455No Hit
ATCGGTTACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAA270.12856530641398028No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTAA270.12856530641398028No Hit
ATCGGTTACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAAC270.12856530641398028No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATCTAGAGGAGCCTGTTCTAAC270.12856530641398028No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG260.12380362839864768No Hit
ATCGGTTACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA250.11904195038331508No Hit
ATCGGTTACTGGGATTAGATACCCCAACCGTCGTTCCACAATCGGCTCAA240.11428027236798247No Hit
ATCGGTTACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAAC240.11428027236798247No Hit
ATCGGTTACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTAAC240.11428027236798247No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT240.11428027236798247No Hit
ATCGGTTACTGGGATTAGATACCCCTGCGTGCCGCGACTAGAGGAGCCTG220.10475691633731728No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.10475691633731728No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA108.4060547E-4295.08
TTACCGG108.4060547E-4295.06
TTACTGG20500.0295.06
TCGGTTA20750.0295.02
GGTTACT20500.0295.04
GGTTACC108.4060547E-4295.04
ATCGGTT20900.0295.01
TACCGGG108.4060547E-4295.07
CTGGATT255.12955E-10295.09
TACTGGG20200.0295.07
CCGGGAT108.4060547E-4295.09
CGGTTAC20650.0295.03
GTTACCG108.4060547E-4295.05
ACTGGGA20250.0294.27168
CTGGGAT20200.0294.26989
GTTACTG20600.0293.567965
ACTGGAT301.5279511E-9245.833318
TACTGGA403.274181E-11221.257
TCGGTTG150.0028298942196.666662
CGAACAA652.1365673E-590.76923295
Produced by FastQC (version 0.11.5)