FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-9-4-2-6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-9-4-2-6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11592
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA209418.064182194616976No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA11179.635955831608005No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTG8997.755348516218081No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5004.313319530710835No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTC4794.13216011042098No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA3973.424775707384403No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2632.268806073153899No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCT2412.0790200138026225No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2382.0531400966183577No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTC2342.018633540372671No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA2121.828847481021394No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1741.5010351966873705No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCT1701.466528640441684No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1701.466528640441684No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCT1641.4147688060731538No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1591.3716356107660457No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCC1381.1904761904761905No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCT1080.9316770186335404No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGT1040.8971704623878536No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1010.8712905452035886No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA990.8540372670807453No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA970.836783988957902No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA730.6297446514837819No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCTC720.6211180124223602No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAG650.5607315389924086No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAGT640.5521048999309869No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTC640.5521048999309869No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA600.5175983436853002No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAG580.500345065562457No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA580.500345065562457No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC570.4917184265010352No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTC540.4658385093167702No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAAGTCT530.4572118702553485No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTC530.4572118702553485No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAG500.4313319530710835No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCT480.4140786749482402No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCTC470.4054520358868185No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAA470.4054520358868185No Hit
GCCACAACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC460.3968253968253968No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAAG450.38819875776397517No Hit
GCCACAACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAAGT450.38819875776397517No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA440.3795721187025535No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAAG430.3709454796411318No Hit
GCCACAACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTCTA420.36231884057971014No Hit
GCCACAACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAAG410.35369220151828845No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA400.3450655624568668No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAAGT370.3191856452726018No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG340.29330572808833677No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAG330.2846790890269151No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA320.27605244996549344No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGTC320.27605244996549344No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA310.26742581090407175No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA300.2587991718426501No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCTC290.2501725327812285No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCTC290.2501725327812285No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCT270.2329192546583851No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA270.2329192546583851No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAA250.21566597653554176No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT250.21566597653554176No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT250.21566597653554176No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAG240.2070393374741201No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAA240.2070393374741201No Hit
GCCACAACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAAGT230.1984126984126984No Hit
GCCACAACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAAGTC220.18978605935127674No Hit
GCCACAACTGGGATTAGATACCCCGTATAGAGGAGCCTGTTCTAAGTCTC220.18978605935127674No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT210.18115942028985507No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC210.18115942028985507No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAA210.18115942028985507No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA210.18115942028985507No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTGACTAGAGGAGCCTGTTCT200.1725327812284334No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA200.1725327812284334No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG190.16390614216701171No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAA180.15527950310559005No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA180.15527950310559005No Hit
GCCACAACTGGGATTAGATACCCCAAGCCCGCGAGACCTAGAGGAGCCTG170.14665286404416839No Hit
GCCACAACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAA160.13802622498274672No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAAGTC150.12939958592132506No Hit
GCCACAACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTAAG150.12939958592132506No Hit
GCCACAACTGGGATTAGATACCCCAACGAGAAAAATTTATCACTTTCCTG130.1121463077984817No Hit
GCCACAACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAAGTCT130.1121463077984817No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAAGTC130.1121463077984817No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTACCTAGAGGAGCCTGTTCTA120.10351966873706005No Hit
GCCACAACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAAGT120.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGG108.3570444E-4295.05
CAACTGG11400.0295.05
AATGGGA108.3570444E-4295.06
GCCACAA11550.0295.01
ACAATGG108.3570444E-4295.04
AACTGGG11400.0293.706126
CTGGGAT11400.0293.706128
ACTGGGA11400.0293.706127
TGGGATT11450.0292.423589
CCACAAC11850.0285.042182
CACAACT12050.0280.311223
ACAACTG12050.0279.087164
ATGGGAT150.002813437196.666667
TATCAAA200.0066521815147.5295
TAAACCT2750.059.00000435-39
GATACCC11450.059.00000415-19
GGATTAG11500.059.00000410-14
GCCAGAG452.5465852E-1159.00000470-74
CCCCGTC452.5465852E-1159.00000420-24
TACCCCT1650.059.00000415-19
Produced by FastQC (version 0.11.5)