FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-89-5-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-89-5-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20505
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA656432.011704462326264No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA15417.515240185320654No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT14687.159229456230188No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG9244.506217995610826No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA7093.4576932455498657No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6883.355279200195074No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA6493.165081687393319No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT3481.6971470373079736No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3321.6191172884662277No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT2441.1899536698366253No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATA2161.0534016093635699No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC2091.019263594245306No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2081.014386734942697No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1720.8388198000487685No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1510.7364057546939771No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1500.7315288953913679No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATAAG1390.6778834430626677No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1380.6730065837600585No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1340.653499146549622No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA1320.6437454279444038No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1260.6144842721287491No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC1240.6047305535235309No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1180.5754693977078762No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCAC1110.5413313825896122No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1090.531577663984394No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGC1060.5169470860765667No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1060.5169470860765667No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT1030.5023165081687393No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAG1020.49743964886613024No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCA970.47305535235308466No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA910.44379419653742985No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC910.44379419653742985No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATA850.41453304072177516No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA830.4047793221165569No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA780.3803950256035113No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA630.30724213606437456No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA630.30724213606437456No Hit
GCCACAACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAT610.29748841745915633No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATAA600.2926115581565472No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAA560.2731041209461107No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT520.25359668373567423No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATA490.2389661058278469No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCA480.23408924652523777No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATAA480.23408924652523777No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG480.23408924652523777No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATA470.2292123872226286No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT460.22433552792001948No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCA420.20482809070958302No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA410.1999512314069739No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCAC400.1950743721043648No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA390.19019751280175565No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAT380.18532065349914653No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATA380.18532065349914653No Hit
GCCACAACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATAA370.18044379419653742No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC360.1755669348939283No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC350.17069007559131918No Hit
GCCACAACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT330.16093635698610095No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT330.16093635698610095No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA320.15605949768349184No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT320.15605949768349184No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAT320.15605949768349184No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCAC260.12679834186783712No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG260.12679834186783712No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCA260.12679834186783712No Hit
GCCACAACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATAAGC250.121921482565228No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTATA240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA240.11704462326261889No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA230.11216776396000974No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA220.10729090465740063No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATAAG220.10729090465740063No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC210.10241404535479151No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.4075064E-4294.965858
AACTGGG19750.0294.965856
ACACTGG206.042319E-8294.965854
CACTGGG206.042319E-8294.965855
CTGGAAT108.4075064E-4294.965858
TGGGTTA108.4075064E-4294.965859
ATTCTTT108.4075064E-4294.96585295
TGGGATA108.4075064E-4294.965859
ACTGGGT108.4075064E-4294.965857
CAACTGG20000.0294.228425
GCCACAA20400.0292.0741
ACTGGGA19900.0292.001377
CTGGGAT19850.0291.99398
TGGGATT19800.0291.241559
CACAACT20850.0284.355563
CCACAAC21000.0283.026762
ACAACTG21100.0280.287454
ACTGGAA150.0028303834196.643897
AACTGGA255.480215E-5176.979526
GCCACAC359.834603E-7168.551931
Produced by FastQC (version 0.11.5)