FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-88-6-4-8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-88-6-4-8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20153
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA993049.27306108271721No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA11325.617029722621942No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA7723.83069518185878No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA6173.061578921252419No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA5822.8879075075671117No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA4942.451247953158339No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA4142.054284721877636No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA4112.0393986007046094No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCAC4112.0393986007046094No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATCGA3921.9451198332754427No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA3481.7267900560710565No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA3201.5878529251228106No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCC3191.5828908847318017No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA3001.488612117302635No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA2541.2603582593162308No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAA2481.2305860169701783No Hit
TGATTGACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTACCA2451.2156998957971519No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCAC2311.1462313303230287No Hit
TGATTGACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGC1910.9477497146826774No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT1780.8832431895995634No Hit
TGATTGACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCA1690.8385848260804842No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCA1640.8137746241254403No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCACT1360.6748374931771944No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCA1250.6202550488760978No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCAC1220.6053689277030715No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACT750.37215302932565875No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT650.3225326254155709No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC550.27291222150548305No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC290.1438991713392547No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA260.12901305016622835No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC240.11908896938421078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC20150.0295.000032
ACTGGGT108.4035244E-4295.09
TGATTGA20200.0294.26981
CACTGGG19950.0294.260658
ACTGGGA19850.0294.256939
ATTGACA20250.0293.54323
ACACTGG20150.0292.8047
GACACTG20300.0292.09366
TTGACAC20300.0292.09364
TGACACT20400.0290.661745
TATGTAA200.006688992147.5295
GCCCTAA13400.059.00000435-39
AGGACCT12200.059.000004105-109
ACCTGGC12300.059.000004110-114
CTTGGCG3050.059.000004110-114
CTGGCGG12350.059.000004110-114
CGGTGCT12950.059.000004115-119
AAGGACC12200.059.000004105-109
TTAAATC11650.059.00000450-54
CCCTAAA13450.059.00000435-39
Produced by FastQC (version 0.11.5)