FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-87-5-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-87-5-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC633431.426445050855868No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA13306.598858843959316No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT12296.097742495658646No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTG8704.316546762589928No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5462.709005209625403No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5082.5204663855122798No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGAT4862.4113123294467873No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATC4352.158273381294964No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGA3981.9746961051848175No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3151.5628876209377325No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2741.3594641528156786No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA2621.2999255767799553No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2511.2453485487472091No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGA2431.2056561647233937No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2331.1560406846936244No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGAT2261.121309848672786No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC1700.843463160506078No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCT1580.7839245844703548No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1490.7392706524435624No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1420.7045398164227239No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1350.6698089804018854No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1230.6102704043661622No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1120.5556933763334161No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1020.5060778963036467No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGA960.47630860828578514No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT900.4465393202679236No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC850.421731580253039No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA830.4118084842470851No Hit
CTGGATGACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA830.4118084842470851No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT780.38700074423220043No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACA740.3671545522202927No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT730.3621930042173158No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT720.3572314562143389No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT690.3423468122054081No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC660.3274621681964773No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT630.31257752418754653No Hit
CTGGATGACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTACA560.27784668816670804No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT550.27288514016373105No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT550.27288514016373105No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACAGAT540.2679235921607541No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACTAGAGGAGCCTGTTCTAC540.2679235921607541No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACAG530.26296204415777724No Hit
CTGGATGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA530.26296204415777724No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC530.26296204415777724No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAC520.2580004961548003No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGAT500.24807740014884647No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT490.2431158521458695No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT480.23815430414289257No Hit
CTGGATGACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTACAGA480.23815430414289257No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC460.22823120813693873No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT460.22823120813693873No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGAT450.2232696601339618No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC430.21334656412800795No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC420.208385016125031No Hit
CTGGATGACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT400.19846192011907715No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGCTAGAGGAGCCTGTTCTACA370.18357727611014635No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGA370.18357727611014635No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG360.17861572810716944No Hit
CTGGATGACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAC350.1736541801041925No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACAG350.1736541801041925No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA340.16869263210121557No Hit
CTGGATGACTGGGATTAGATACCCCTGACGTGCTAGAGGAGCCTGTTCTA340.16869263210121557No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA320.15876953609526173No Hit
CTGGATGACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC310.15380798809228477No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAG300.14884644008930786No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAG290.14388489208633093No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT290.14388489208633093No Hit
CTGGATGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACA280.13892334408335402No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT270.13396179608037706No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGAT270.13396179608037706No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACA260.12900024807740015No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA260.12900024807740015No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA250.12403870007442323No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGAT230.11411560406846936No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT230.11411560406846936No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG230.11411560406846936No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC210.1041925080625155No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT210.1041925080625155No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAAC210.1041925080625155No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG108.4035244E-4295.06
GATGGGA108.4035244E-4295.07
ATGGGAT108.4035244E-4295.08
GACTGGG19700.0295.07
GATGACT19900.0294.25884
ATGACTG19850.0294.256935
TGACTGG19800.0294.255046
ACTGGGA19750.0294.253178
CTGGATG20150.0293.5361
CTGGGAT19750.0293.506329
TGGATGA20200.0292.079222
GGATGAC20100.0292.064673
ACTAACC150.0028290446196.66666295
ATGATGG150.0028290446196.666665
GCAACTA301.1325224E-4147.49998295
GATACCC20100.059.00000415-19
CGCCAGA8100.059.00000470-74
GGGATTA19700.059.00000410-14
TGGCGGT10050.059.000004110-114
ATACCCC20100.059.00000415-19
Produced by FastQC (version 0.11.5)