FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-86-4-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-86-4-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20296
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA290414.30823807646827No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC256612.642885297595585No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCT19399.553606621994481No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACT9894.872881355932203No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA7953.9170279858100123No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA6533.217382735514387No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCAC5052.4881750098541584No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCAC4852.389633425305479No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT4422.177769018525818No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACT3491.719550650374458No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC3171.5618841150965708No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2711.3352384706346077No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC2391.1775719353567204No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCC2371.1677177769018527No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2341.1529365392195507No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1870.9213638155301537No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1770.872093023255814No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACT1710.8425305478912101No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTC1640.8080409932991722No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC1640.8080409932991722No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1630.8031139140717384No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCA1610.7932597556168702No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCAC1460.7193535672053606No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC1420.6996452502956247No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC1380.6799369333858889No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1370.6750098541584548No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA1330.655301537248719No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCA1220.6011036657469452No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACT1120.5518328734726055No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1080.5321245565628695No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACC1020.5025620811982657No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1010.49763500197083166No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCAC1010.49763500197083166No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA1000.4927079227433977No Hit
CTGGATGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACC990.48778084351596374No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA970.47792668506109576No Hit
CTGGATGACTGGGATTAGATACCCCTGTAACTAGAGGAGCCTGTTCTACC970.47792668506109576No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAC900.44343713046905797No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC810.39909341742215215No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAC750.3695309420575483No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT750.3695309420575483No Hit
CTGGATGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAC700.3448955459203784No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACT690.33996846669294445No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC680.33504138746551043No Hit
CTGGATGACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACC660.3251872290106425No Hit
CTGGATGACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACCA650.32026014978320855No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACT650.32026014978320855No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT620.30547891210090655No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT620.30547891210090655No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA610.3005518328734726No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC570.2808435159637367No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT560.27591643673630273No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT540.26606227828143475No Hit
CTGGATGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTACC530.2611351990540008No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACCA510.25128104059913287No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACT480.2364998029168309No Hit
CTGGATGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTAC470.23157272368939694No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT470.23157272368939694No Hit
CTGGATGACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTACC460.22664564446196295No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT450.22171856523452899No Hit
CTGGATGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT440.21679148600709497No Hit
CTGGATGACTGGGATTAGATACCCCCACGGACCTAGAGGAGCCTGTTCTA410.20201024832479306No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACT400.1970831690973591No Hit
CTGGATGACTGGGATTAGATACCCCGCCACGAGACCTAGAGGAGCCTGTT400.1970831690973591No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAC390.1921560898699251No Hit
CTGGATGACTGGGATTAGATACCTCACGAGACCTAGAGGAGCCTGTTCTA380.18722901064249114No Hit
CTGGATGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACC380.18722901064249114No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACTAGAGGAGCCTGTTCTAC360.17737485218762317No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT330.16259361450532125No Hit
CTGGATGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAC320.15766653527788727No Hit
CTGGATGACTGGGATTAGATACCCCCCGACCTAGAGGAGCCTGTTCTACC320.15766653527788727No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACC300.14781237682301931No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT300.14781237682301931No Hit
CTGGATGACTGGGATTAGATACCCCTAGATCGGAAGAGCGCTAGAGGAGC300.14781237682301931No Hit
CTGGATGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTACCA290.14288529759558535No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC290.14288529759558535No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCGACTAGAGGAGCCTGTTCT270.13303113914071737No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT270.13303113914071737No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT260.1281040599132834No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC260.1281040599132834No Hit
CTGGATGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA230.11332282223098147No Hit
CTGGATGACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTAC220.10839574300354748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC20100.0295.03
TGACTGG20100.0295.06
CTGGATT108.4038323E-4295.09
CTGGATG20150.0295.01
GACTGGG20000.0294.262487
TGGATGA20250.0293.54322
CTGGGAT20000.0293.5259
ACTGGGA20000.0293.5258
ATGACTG20250.0292.814825
GATGACT20300.0292.09364
GACTGGA202.2492848E-5221.257
ACTGGAT255.4767E-5177.08
CAGCCCT150.004368995359.00000430-34
CCGTGTA303.3119977E-759.00000420-24
TTACCGA257.78297E-659.00000455-59
CCCGGTA501.8189894E-1259.00000420-24
CCTGTAG1000.059.00000420-24
CCGCCTA150.004368995359.00000420-24
CATCCCT150.004368995359.000004125-129
AGGGCCT501.8189894E-1259.00000435-39
Produced by FastQC (version 0.11.5)