FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-85-4-8-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-85-4-8-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20304
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA388219.1193853427896No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC15567.663514578408195No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG13616.703112687155241No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA10795.314223798266352No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG8464.166666666666666No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA7613.7480299448384553No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG4752.3394405043341213No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGA3911.9257289204097714No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3751.8469267139479904No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3651.7976753349093777No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA3441.69424743892829No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA3131.5415681639085894No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTG3061.5070921985815602No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2771.3642631993695824No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2761.3593380614657211No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2631.295311268715524No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGAT2471.2165090622537431No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTG2321.1426319936958234No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2221.0933806146572105No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA2201.0835303388494877No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1940.9554767533490938No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1860.9160756501182032No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAG1850.911150512214342No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1540.7584712371946414No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGATT1230.6057919621749409No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1110.5466903073286052No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1070.52698975571316No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG1020.5023640661938534No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT990.4875886524822695No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTG940.4629629629629629No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA890.43833727344365647No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGA800.3940110323089046No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA790.3890858944050433No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTGA770.3792356185973207No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATT730.3595350669818755No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTGA710.34968479117415285No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTGA710.34968479117415285No Hit
CAACACACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTAGATTG710.34968479117415285No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG640.31520882584712373No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT640.31520882584712373No Hit
CAACACACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCTA640.31520882584712373No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG620.3053585500394011No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGA620.3053585500394011No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA620.3053585500394011No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGA600.29550827423167847No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG590.2905831363278172No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA570.28073286052009455No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA570.28073286052009455No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA560.27580772261623326No Hit
CAACACACTGGGATTAGATACCCCCGCAATCTAGAGGAGCCTGTTCTAGA540.26595744680851063No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA510.2511820330969267No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC510.2511820330969267No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGATTG480.2364066193853428No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT480.2364066193853428No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCT430.21178092986603625No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGA420.20685579196217493No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGATTG410.20193065405831362No Hit
CAACACACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTAG400.1970055161544523No Hit
CAACACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAG380.18715524034672973No Hit
CAACACACTGGGATTAGATACCCCACGTGATCTAGAGGAGCCTGTTCTAG380.18715524034672973No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA380.18715524034672973No Hit
CAACACACTGGGATTAGATACCCCTGACTGCCAACTAGAGGAGCCTGTTC370.1822301024428684No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA350.17237982663514578No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA340.16745468873128447No Hit
CAACACACTGGGATTAGATACCCCGAACTAGAGGAGCCTGTTCTAGATTG340.16745468873128447No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGATTG330.16252955082742315No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA330.16252955082742315No Hit
CAACACACTGGGATTAGATACCCCGTTCTAGAGGAGCCTGTTCTAGATTG330.16252955082742315No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGA320.15760441292356187No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCTCTAGAGGAGCCTGTTCTA300.14775413711583923No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA300.14775413711583923No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG290.14282899921197792No Hit
CAACACACTGGGATTAGATACCCCGTCCAGAGGAGCCTGTTCTAGATTGA280.13790386130811663No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA270.13297872340425532No Hit
CAACACACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAGATTG270.13297872340425532No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG260.128053585500394No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGA260.128053585500394No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCTG260.128053585500394No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT250.12312844759653271No Hit
CAACACACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCT240.1182033096926714No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGAT230.11327817178881008No Hit
CAACACGCTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC230.11327817178881008No Hit
CAACACACTGGGATTAGATACCCCGGCTAGAGGAGCCTGTTCTAGATTGA210.10342789598108747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG108.403986E-4295.06
ACGCTGG108.403986E-4295.05
GCTGGGA108.403986E-4295.07
CACTGGG19700.0295.06
ACACTGG19900.0294.25885
ACTGGGA19700.0294.251287
CTGGGAT19850.0293.513858
TGGGATT19850.0293.513859
CACACTG20150.0292.071964
ACACACT21800.0269.96563
CAACACA24500.0241.418371
AACACAC25950.0227.36032
CTGGATT202.2493668E-5221.258
TGGATTA202.2493668E-5221.259
ACTGGAT202.2493668E-5221.257
CACGCTG150.0028291994196.666674
TAATTTA150.0028291994196.66667295
CACTGGA301.13264614E-4147.56
CAGCCCT257.783108E-659.00000430-34
AACGCTT150.00436902659.00000490-94
Produced by FastQC (version 0.11.5)