FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-84-5-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-84-5-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19480
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG598330.713552361396303No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACA14867.628336755646817No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG12416.370636550308008No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGTC7383.7885010266940453No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATCT6883.5318275154004106No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC5722.9363449691991788No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4942.5359342915811087No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGATC3451.7710472279260778No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTGT2641.3552361396303902No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2501.2833675564681724No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2391.226899383983573No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACA2391.226899383983573No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAGA2341.2012320328542094No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACA2301.1806981519507187No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1940.9958932238193019No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCTG1940.9958932238193019No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGATC1870.9599589322381931No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC1830.9394250513347022No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATCT1640.8418891170431212No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1500.7700205338809034No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACAG1470.7546201232032854No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGATCT1450.74435318275154No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC1380.7084188911704312No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1240.6365503080082136No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGATC1000.5133470225872689No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG960.49281314168377827No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC950.4876796714579056No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC950.4876796714579056No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG890.4568788501026694No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGATC860.4414784394250513No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT840.431211498973306No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG740.3798767967145791No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG740.3798767967145791No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG700.3593429158110883No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACAG690.3542094455852156No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATCT630.32340862422997946No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACA620.3182751540041068No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT600.3080082135523614No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG590.3028747433264887No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTACAGAT580.297741273100616No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACAG570.2926078028747433No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG570.2926078028747433No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGAT540.27720739219712526No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT520.2669404517453799No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGATCT520.2669404517453799No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT510.2618069815195072No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT500.25667351129363447No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTACA490.2515400410677618No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC490.2515400410677618No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACAGATCTG460.23613963039014374No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCCCTAGAGGAGCCTGTTCTACA450.23100616016427106No Hit
AAGCGACTGGGATTAGATACCCCTACGAGACCTAGAGGAGCCTGTTCTAC450.23100616016427106No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT450.23100616016427106No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACAG450.23100616016427106No Hit
AAGCGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTAC440.22587268993839835No Hit
AAGCGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTACAG440.22587268993839835No Hit
AAGCGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTACAGAT420.215605749486653No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTACA400.20533880903490762No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACAG400.20533880903490762No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACAGATC390.2002053388090349No Hit
AAGCGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTACAG350.17967145790554415No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAGAT350.17967145790554415No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGATCT350.17967145790554415No Hit
AAGCGACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTACA340.17453798767967144No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG330.16940451745379878No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA310.1591375770020534No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACA310.1591375770020534No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT310.1591375770020534No Hit
AAGCGACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTACA300.1540041067761807No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACAGA290.148870636550308No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG280.1437371663244353No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACAGATC280.1437371663244353No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCAGACCCTAGAGGAGCCTG270.13860369609856263No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG240.12320328542094457No Hit
AAGCGACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTACAGA230.11806981519507187No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA230.11806981519507187No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT230.11806981519507187No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGATCT220.11293634496919917No Hit
AAGCGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACAGAT220.11293634496919917No Hit
AAGCGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTACAG210.1078028747433265No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG210.1078028747433265No Hit
AAGCGACTGGGATTAGATACCCCCGTGCTAGAGGAGCCTGTTCTACAGAT210.1078028747433265No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACAG210.1078028747433265No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTACA200.10266940451745381No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG200.10266940451745381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG19400.0295.03
CTGGGAT19300.0295.07
TGGGATT19250.0295.08
AGCGACT19400.0295.02
GACTGGG19350.0294.999975
ACTGGGA19400.0294.23976
GGGATTA19300.0294.235759
AAGCGAC19550.0293.491061
CGACTGG19500.0293.487184
CTGACAA150.0028282893196.66666295
TCCACAA150.0028282893196.66666295
AAACTTT452.5465852E-1159.00000435-39
TATGCCT2750.059.00000425-29
CCGCCAG2750.059.00000465-69
TAGCCCT7950.059.00000430-34
CAATTGA1550.059.00000455-59
AACCTTG1550.059.00000440-44
AACTTTG452.5465852E-1159.00000440-44
ACTTTGA452.5465852E-1159.00000440-44
TATCCGC2750.059.00000465-69
Produced by FastQC (version 0.11.5)