FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-83-4-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-83-4-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19619
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG382719.506600744176563No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAG243912.431826290840512No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTGT13246.748560069320557No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG7803.9757378051888477No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTCC6493.308017737907131No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4422.2529180896070136No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAG4042.0592282990978132No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCCT3671.8706356083388553No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3261.6616545185789287No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCTC3181.6208777205769915No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCTC3161.6106835210765076No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA3111.585198022325297No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAG2731.3915082318160967No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2661.355828533564402No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2351.1978184413068964No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGTCT2301.1723329425556859No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTCC2261.1519445435547175No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA2231.1366532443039912No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGTC2081.0601967480503593No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1860.9480605535450329No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1810.9225750547938224No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAG1690.8614098577909169No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCTC1630.8308272592894643No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA1560.7951475610377696No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCTG1440.7339823640348642No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1150.5861664712778429No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAGT1130.5759722717773588No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGTCT1100.5606809725266324No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1080.5504867730261481No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAGT1070.5453896732759059No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA1060.5402925735256638No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAGT910.4638360772720322No Hit
AAGCGACTGGGATTAGATACCCCTGACCTGCCGCTAGAGGAGCCTGTTCT870.44344767827106374No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAGT860.43835057852082165No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT850.4332534787705795No Hit
AAGCGACTGGGATTAGATACCCCTGTGCTAGAGGAGCCTGTTCTAAGTCT840.42815637902033743No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCTCC820.41796217951985315No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTCC740.3771853815179163No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAGT730.37208828176767417No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAGT690.3516998827667058No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG680.34660278301646363No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCTC660.3364085835159794No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAGT650.33131148376573727No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTCC640.3262143840154952No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTAA620.31602018451501096No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG610.3109230847647688No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACAAGACCTAGAGGAGCCTGT610.3109230847647688No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG610.3109230847647688No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG580.2956317855140425No Hit
AAGCGACTGGGATTAGATACCCCTGACGCATAGAGGAGCCTGTTCTAAGT550.2803404862633162No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG540.27524338651307406No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCCCTAGAGGAGCCTGTTCTAAG530.2701462867628319No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTCC530.2701462867628319No Hit
AAGCGACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAAGTCT520.26504918701258984No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCCT520.26504918701258984No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT510.2599520872623477No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCTC470.23956368826137928No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT470.23956368826137928No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAAGT470.23956368826137928No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG440.22427238901065297No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAA440.22427238901065297No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAAGTCT440.22427238901065297No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG430.21917528926041083No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCTAGAGGAGCCTGTTCTAAGTC430.21917528926041083No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG430.21917528926041083No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA420.21407818951016871No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCTG410.20898108975992657No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA410.20898108975992657No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAAG390.1987868902594424No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCTCC390.1987868902594424No Hit
AAGCGACTGGGATTAGATACCCCATACGACCCACTAGAGGAGCCTGTTCT370.18859269075895815No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAAG340.17330139150823182No Hit
AAGCGACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAAGTCTC330.1682042917579897No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAGTCT330.1682042917579897No Hit
AAGCGACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAAGTCTC310.15801009225750548No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCTCC310.15801009225750548No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT300.15291299250726337No Hit
AAGCGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT300.15291299250726337No Hit
AAGCGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAAGTCT290.14781589275702126No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG290.14781589275702126No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAAG280.14271879300677914No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAGTC270.13762169325653703No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCTCC270.13762169325653703No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG270.13762169325653703No Hit
AAGCGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAAGT260.13252459350629492No Hit
AAGCGACTGGGATTAGATACCCCTGGTGACGACTAGAGGAGCCTGTTCTA250.1274274937560528No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC250.1274274937560528No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC230.11723329425556857No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG200.10194199500484225No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG200.10194199500484225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG206.031951E-8295.03
GACGGGA206.031951E-8295.05
CGGGATT206.031951E-8295.07
ACGGGAT206.031951E-8295.06
GGGATAG108.40177E-4295.09
CGACGGG206.031951E-8295.04
TGGGATA108.40177E-4295.08
CGACTGG19200.0295.04
CTGGGAT19150.0294.999977
GACTGGG19150.0294.999975
ACTGGGA19200.0294.231786
GCGACTG19350.0293.475463
TGGGATT19150.0293.45958
AGCGACT19400.0292.71912
GGGATTA19350.0290.426369
AAGCGAC19850.0289.055421
AGCGACG251.8357059E-7236.02
CAGCCCT150.004368576759.00000430-34
GCCCCAA452.5465852E-1159.000004115-119
GGAGATC501.8189894E-1259.00000425-29
Produced by FastQC (version 0.11.5)