FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-82-5-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-82-5-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC522327.184718680060378No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA18309.524800916046427No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA12276.3863009420704735No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT10645.537917035340654No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTG7333.8151251756623124No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4912.5555613386769376No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4642.4150314890959246No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT4422.300525685733618No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3551.847707281528132No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2891.5041898714412116No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2791.4521417790037996No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT2601.3532504033727164No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAG2401.249154218497892No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2261.176286889085515No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2201.1450580336230678No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2161.124238796648103No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGA1750.9108416176547128No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1670.8692031437047834No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTG1550.8067454327798886No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATT1450.7546973403424764No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATT1420.7390829126112528No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1240.645396346223911No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1030.5360953521053454No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATT1030.5360953521053454No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG1000.5204809243741216No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG980.5100713058866393No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGAT950.4944568781554156No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT940.4892520689116744No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT910.47363764118045076No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCTACTAGAGGAGCCTGTTCT890.4632280226929683No Hit
CTGGATGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGA810.4215895487430386No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG750.3903606932805913No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT740.3851558840368501No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT740.3851558840368501No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA720.37474626554936763No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA720.37474626554936763No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTG680.3539270285744028No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC640.3331077915994379No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT620.3226981731119555No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC600.312288554624473No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC600.312288554624473No Hit
CTGGATGACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCT590.3070837453807318No Hit
CTGGATGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGAT590.3070837453807318No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTG510.26544527143080204No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT510.26544527143080204No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT480.2498308436995784No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGA450.23421641596835474No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT450.23421641596835474No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTG430.22380679748087232No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTG430.22380679748087232No Hit
CTGGATGACTGGGATTAGATACCCCTGGACCTAGAGGAGCCTGTTCTAGA340.1769635142872014No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGAT330.17175870504346016No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA320.16655389579971894No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA320.16655389579971894No Hit
CTGGATGACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAG320.16655389579971894No Hit
CTGGATGACTGGGATTAGATACCCCTCCAAGCCCACGAGACCTAGAGGAG270.14052984958101286No Hit
CTGGATGACTGGGATTAGATACCCCACCACCTAGAGGAGCCTGTTCTAGA260.13532504033727163No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA250.1301202310935304No Hit
CTGGATGACTGGGATTAGATACCCCTGTGGACTAGAGGAGCCTGTTCTAG250.1301202310935304No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT250.1301202310935304No Hit
CTGGATGACTGGGATTAGATACCCCGACGTGCCTAGAGGAGCCTGTTCTA240.1249154218497892No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG230.11971061260604798No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC230.11971061260604798No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG230.11971061260604798No Hit
CTGGATGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGA220.11450580336230677No Hit
CTGGATGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG220.11450580336230677No Hit
CTGGATGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCC200.10409618487482433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG157.130224E-6295.06
GATGGGA157.130224E-6295.07
ATGGGAT157.130224E-6295.08
ATGATGG157.130224E-6295.05
CTGGATT157.130224E-6295.09
GACTGGG18600.0295.07
GACGGGA108.400433E-4294.999977
CGGGATT108.400433E-4294.999979
CTGGATA108.400433E-4294.999971
ACGGGAT108.400433E-4294.999978
TGACGGG108.400433E-4294.999976
CTGGATG19150.0294.229741
TGGATGA19150.0294.229742
TGACTGG18850.0294.21756
CTGGGAT18650.0294.209149
GGATGAC19050.0293.451453
ACTGGGA18700.0293.422458
GATGACT19000.0292.671054
ATGACTG19100.0291.138765
GGATGAT202.247466E-5221.249983
Produced by FastQC (version 0.11.5)