FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-81-4-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-81-4-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19178
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG342117.83814787777662No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA232312.112837626446971No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT18149.458754823234957No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGAG8114.228803837730733No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG7203.7543018041505896No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA5142.6801543435186153No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA3611.8823652101366148No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC3341.7415788924809679No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGAC3311.7259359682970072No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG3001.564292418396079No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2741.428720408801752No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTGAG2691.4026488684951508No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG2631.371363020127229No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTGA2621.3661487120659088No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTGA2281.1888622379810199No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2241.168005005735739No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAT2211.152362081551778No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAGA1900.9907185316508499No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGATT1790.933361142976327No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTGA1570.8186463656272812No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGAG1530.7977891333820002No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTGA1460.7612889769527583No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1420.7404317447074773No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1380.7195745124621963No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGAT1320.6882886640942747No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1310.6830743560329544No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAT1210.6309312754197518No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG1130.5892168109291898No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1000.5214308061320263No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGA940.4901449577641047No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG890.46407341745750336No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGA860.4484304932735426No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA840.4380018771509021No Hit
AAGCGACTGGGATTAGATACCCCACAAGGCCTAGAGGAGCCTGTTCTAGA800.41714464490562103No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGAG790.41193033684430075No Hit
AAGCGACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGAT750.3910731045990197No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG740.38585879653769944No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG740.38585879653769944No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGAT720.37543018041505893No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG710.37021587235373865No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGA700.36500156429241837No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAT610.31807279174053604No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG600.31285848367921576No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGATTG590.3076441756178955No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGAT590.3076441756178955No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG570.29721555949525497No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAGA560.29200125143393474No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC560.29200125143393474No Hit
AAGCGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGATT520.27114401918865366No Hit
AAGCGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC520.27114401918865366No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT520.27114401918865366No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAGA500.26071540306601315No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG500.26071540306601315No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGATTG500.26071540306601315No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG490.25550109500469287No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAGA450.23464386275941185No Hit
AAGCGACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCCT450.23464386275941185No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCACGAGACCTAGAGGAGCCTGT440.22942955469809154No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGAG430.2242152466367713No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGATTG430.2242152466367713No Hit
AAGCGACTGGGATTAGATACCCCGATGTCCAGAGGAGCCTGTTCTAGATT420.21900093857545105No Hit
AAGCGACTGGGATTAGATACCCCACGAGAGCTAGAGGAGCCTGTTCTAGA400.20857232245281052No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG400.20857232245281052No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG380.19814370633016998No Hit
AAGCGACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGATTG370.19292939826884972No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT350.18250078214620918No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGAG340.17728647408488893No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT320.16685785796224842No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGATTG310.16164354990092814No Hit
AAGCGACTGGGATTAGATACCGACGAGAGGAGCCTGTTCTAGATTGAGAC310.16164354990092814No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA310.16164354990092814No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGATTG310.16164354990092814No Hit
AAGCGACTGGGATTAGATACCTCGAGCCACGAGACCTAGAGGAGCCTGTT300.15642924183960788No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC300.15642924183960788No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG290.15121493377828762No Hit
AAGCGACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCTAG280.14600062571696737No Hit
AAGCGACTGGGATTAGATACCCCAACCAACCTAGAGGAGCCTGTTCTAGA270.1407863176556471No Hit
AAGCGACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGATTGAG270.1407863176556471No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTGAG260.13557200959432683No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT260.13557200959432683No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTGAG250.13035770153300658No Hit
AAGCGACTGGGATTAGATACCCCTGATGCCGACTAGAGGAGCCTGTTCTA240.12514339347168632No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG240.12514339347168632No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT240.12514339347168632No Hit
AAGCGACTGGGATTAGATACCCCCGACTAGAGGAGCCTGTTCTAGATTGA230.11992908541036604No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGAT220.11471477734904577No Hit
AAGCGACTGGGATTAGATACCCCTTATACACATCTGACGCTAGAGGAGCC210.10950046928772553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT18850.0295.07
GACTGGG18900.0295.05
GGGATAG108.4002607E-4295.09
TGGGATA108.4002607E-4295.08
ACTGGGA18850.0295.06
CGACTGG19050.0295.04
GACTGGA157.1299346E-6294.999975
AAGCGAC19150.0294.229771
TGGGATT18800.0294.215428
GCGACTG19150.0293.459533
AGCGACT19150.0293.459532
GGGATTA18850.0292.65259
TGGATTA202.2473741E-5221.258
ACTGGAT202.2473741E-5221.256
TCGATAA200.0066864076147.5295
CACGTAA301.1316465E-4147.49998295
CTGGATT301.1316465E-4147.499987
GCCTCAG1500.059.000004115-119
CGCCAGC1250.059.00000485-89
GCCAGGG1250.059.00000470-74
Produced by FastQC (version 0.11.5)