FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-80-4-1-3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-80-4-1-3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19310
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC336317.415846711548422No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA18209.425168306576904No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGC16668.627654065251166No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAAT9404.867944070429829No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT7623.9461418953909893No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6683.4593474883480058No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCG4282.216468151216986No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAA3771.9523562920766444No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAA3751.9419989642672193No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3511.817711030554117No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3261.6882444329363022No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3101.605385810460901No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAAT2821.4603832211289487No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2731.4137752459865354No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2451.2687726566545832No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2131.1030554117037805No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1941.0046607975142414No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1910.9891248058001036No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1860.9632314862765408No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1560.8078715691351631No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATC1520.7871569135163128No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1510.7819782496116001No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1350.6991196271361988No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGG1330.6887622993267737No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA1230.6369756602796478No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1050.5437597099948214No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAA920.47643707923355777No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA920.47643707923355777No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT810.4194717762817193No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC810.4194717762817193No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGG760.3935784567581564No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGG760.3935784567581564No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATC720.37286380113930606No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC690.3573278094251683No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT690.3573278094251683No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG610.3158984981874676No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA590.30554117037804246No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC590.30554117037804246No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAAT590.30554117037804246No Hit
ATCGGTTACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTA570.2951838425686173No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT570.2951838425686173No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT560.29000517866390474No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG550.28482651475919213No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT550.28482651475919213No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGA530.27446918694976696No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGGA510.2641118591403418No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT510.2641118591403418No Hit
ATCGGTTACTGGGATTAGATACCCCGATCCCACGAGACCTAGAGGAGCCT500.25893319523562924No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGA500.25893319523562924No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT500.25893319523562924No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT490.25375453133091663No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAG490.25375453133091663No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG480.24857586742620405No Hit
ATCGGTTACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAGGAAT480.24857586742620405No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC470.24339720352149147No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAAT460.23821853961677888No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAAT460.23821853961677888No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGGA440.22786121180735372No Hit
ATCGGTTACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGGA420.21750388399792853No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGACTAGAGGAGC420.21750388399792853No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT420.21750388399792853No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT400.20714655618850336No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGGAACTAGAGGAGCCTGTT390.20196789228379078No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAA390.20196789228379078No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGGA390.20196789228379078No Hit
ATCGGTTACTGGGATTAGATACCCCACGATCTAGAGGAGCCTGTTCTAGG380.1967892283790782No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCACGACCTAGAGGAGCCTG380.1967892283790782No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG370.1916105644743656No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG360.18643190056965303No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT350.18125323666494045No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGGA340.17607457276022787No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGA340.17607457276022787No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT340.17607457276022787No Hit
ATCGGTTACTGGGATTAGATACCCCCTGTAGTCCCTAGAGGAGCCTGTTC320.1657172449508027No Hit
ATCGGTTACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA320.1657172449508027No Hit
ATCGGTTACTGGGATTAGATACCCCTGTGTATAAGAGACAGCCACGTCTA310.16053858104609012No Hit
ATCGGTTACTGGGATTAGATACCCCAGGTAGCTAGAGGAGCCTGTTCTAG310.16053858104609012No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC300.15535991714137753No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGGCCTAGAGGAGCCTGTTCTAG290.15018125323666495No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG280.14500258933195237No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGAGGAGCCTGTTCTAGGAATC280.14500258933195237No Hit
ATCGGTTACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGGA280.14500258933195237No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTAGGA270.1398239254272398No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAAT270.1398239254272398No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGGAAT260.1346452615225272No Hit
ATCGGTTACTGGGATTAGATACCCCCGACTAGAGGAGCCTGTTCTAGGAA260.1346452615225272No Hit
ATCGGTTACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAGGAA250.12946659761781462No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGGAAT250.12946659761781462No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT240.12428793371310203No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGA240.12428793371310203No Hit
ATCGGTTACTGGGATTAGATACCCCCGGATCTAGAGGAGCCTGTTCTAGG240.12428793371310203No Hit
ATCGGTTACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGGAAT230.11910926980838944No Hit
ATCGGTTACTGGGATTAGATACCCCATATGTGCAGGGAGGGTACCTTGAG230.11910926980838944No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCT220.11393060590367686No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA210.10875194199896426No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT200.10357327809425168No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA200.10357327809425168No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA108.4007723E-4295.08
CTGGGTT206.029586E-8295.09
TTACTGG19000.0295.06
CTGGGAT18600.0295.09
TCGGTTA19250.0295.02
GGTTACT19050.0295.04
GGTTACC108.4007723E-4295.04
ATCGGTT19250.0295.01
TACCGGG108.4007723E-4295.07
TACTGGG18850.0295.07
CCGGGAT108.4007723E-4295.09
CGGTTAC19200.0295.03
ACTGGGT206.029586E-8295.08
ACTGGGA18650.0295.08
GTTACCG108.4007723E-4295.05
GTTACTG19050.0294.225745
TTACCGG150.0028281203196.666676
CTGGATT150.0028281203196.666679
TACTGGA255.47272E-5177.07
ACTGGAT255.47272E-5177.08
Produced by FastQC (version 0.11.5)