FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-8-2-1-3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-8-2-1-3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11138
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG123411.079188364158735No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG6285.638355180463279No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG6095.467768001436523No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG4243.806787574070749No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3473.115460585383372No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG3222.891003770874484No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG2882.5857425031423955No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG2702.4241335966959956No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG2582.316394325731729No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG2201.9752199676782187No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG2011.8046327886514633No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2001.7956545160711082No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG1781.5981325193032863No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG1691.5173280660800863No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG1651.4814149757586639No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG1531.3736757047943975No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1481.3287843418926197No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG1461.3108277967319086No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG1211.0863709822230203No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG1161.0414796193212426No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1121.0055665289998204No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG1110.9965882564194648No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG1110.9965882564194648No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG1100.9876099838391094No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1090.9786317112587537No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG980.8798707128748428No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG970.8708924402944873No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG890.7990662596516431No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG850.7631531693302208No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG850.7631531693302208No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT790.7092835338480876No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG790.7092835338480876No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG760.682348716107021No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG730.6554138983659543No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG710.6374573532052433No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG680.6105225354641767No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG630.5656311725623989No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG620.5566528999820434No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG620.5566528999820434No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG610.5476746274016879No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG610.5476746274016879No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG600.5386963548213324No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG580.5207398096606213No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG510.45789190159813253No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG510.45789190159813253No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG490.4399353564374214No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG480.4309570838570659No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG470.42197881127671033No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG450.4040222661159993No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG390.35015263063386604No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG390.35015263063386604No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG380.3411743580535105No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG370.33219608547315493No Hit
ATCGGTTGTGTCAGCAGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT290.26036990483031064No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG290.26036990483031064No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTATTCG290.26036990483031064No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGGTCCAAGCGTTATCCG280.2513916322499551No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG270.24241335966959957No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCG260.23343508708924404No Hit
ATCGGTTGTGTCAGCAGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG250.22445681450888852No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG250.22445681450888852No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCG250.22445681450888852No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG230.2065002693481774No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCG230.2065002693481774No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA230.2065002693481774No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCG200.1795654516071108No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG200.1795654516071108No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG190.17058717902675524No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT180.16160890644639972No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACAGGGGATGCAAGCGTTATCCG170.15263063386604417No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG170.15263063386604417No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCG160.14365236128568865No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTATTCG160.14365236128568865No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCG160.14365236128568865No Hit
ATCGGTTGTGCCAGCCGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG160.14365236128568865No Hit
ATCGGTTGTGTCAGCCGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG150.1346740887053331No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGAAGGTCCCGAGCGTTATTCG140.12569581612497754No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCG140.12569581612497754No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTATTCG130.11671754354462202No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG130.11671754354462202No Hit
ATCGGTTGTGCCAGCCGCCGCGGTAATACAGAGGATGCAAGCGTTATCCG130.11671754354462202No Hit
ATCGGTTGTGTCAGCCGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA130.11671754354462202No Hit
ATCGGTTGTGTCAGCAGCCGCGGTAAGACGGGGGATGCAAGTGTTATCCG120.10773927096426647No Hit
ATCGGTTGTGCCAGCCGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT120.10773927096426647No Hit
ATCGGTTGTGCCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG120.10773927096426647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTC108.3553087E-4294.96848295
TCGGTTA157.0521583E-6294.968482
AGTACTT108.3553087E-4294.96848295
AGGCCTT205.9179E-8294.96848295
AGTCCTC1600.0294.96848295
AGTCCTA108.3553087E-4294.96848295
AGTCCAC157.0521583E-6294.96848295
CGGTTAC157.0521583E-6294.968483
AGTCATT108.3553087E-4294.96848295
AATCCTT350.0294.96844295
ATCGGTT11100.0294.968441
TTGTGCC3300.0294.968446
TGTGCCA3300.0294.968447
TCGGTTG11000.0293.627722
TTGTGTC7600.0293.027866
GTTGTGT7600.0293.027865
TGTCAGC7550.0293.015059
GGTTGTG10950.0292.274724
CGGTTGT10950.0292.274723
TGCCAGC3350.0290.565959
Produced by FastQC (version 0.11.5)