FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-79-5-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-79-5-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18839
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC256413.610064228462232No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA248613.196029513243804No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGC16638.827432453951909No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAAT8564.543765592653537No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAA6193.2857370348744626No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC4812.5532140771803173No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4692.4895164286851745No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT4382.3249641700727213No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCG4092.171028186209459No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3882.0595573013429584No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATC3691.9587026912256489No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT3401.8047667073623863No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2971.5765168002547907No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAAT2781.4756621901374807No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAA2541.3482668931471946No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2211.173098359785551No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2211.173098359785551No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGGA2201.167790222410956No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1971.045703062795265No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGA1911.0138542385476936No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1880.9979298264239078No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGG1580.8386857051860501No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGG1500.7962206061892882No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1280.6794415839481925No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG1230.6529008970752163No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1110.5892032485800732No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG1020.5414300122087159No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG990.5255056000849302No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA900.47773236371357286No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAAT870.4618079515897871No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAA860.45649981421519187No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAAT860.45649981421519187No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT860.45649981421519187No Hit
CTGGATGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA810.4299591273422156No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAAT800.4246509899676203No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAAT760.40341844046923936No Hit
CTGGATGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGG700.3715696162216678No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT690.36626147884707255No Hit
CTGGATGACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA660.3503370667232868No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGA650.34502892934869156No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC640.33972079197409627No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG620.32910451722490575No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT600.3184882424757153No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT550.291947555602739No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA530.28133128085354847No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT530.28133128085354847No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG510.27071500610435795No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC480.2547905939805722No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC470.24948245660597695No Hit
CTGGATGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGGAA460.2441743192313817No Hit
CTGGATGACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGA460.2441743192313817No Hit
CTGGATGACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAGGAA450.23886618185678643No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCGACGACTAGAGGAGCCTGT430.22824990710759593No Hit
CTGGATGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGG430.22824990710759593No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT420.22294176973300067No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGA420.22294176973300067No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTTAGAGGAGCCTGTTCT400.21232549498381015No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT380.20170922023461968No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG380.20170922023461968No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG380.20170922023461968No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT360.19109294548542916No Hit
CTGGATGACTGGGATTAGATACCCCAACTTAGAGGAGCCTGTTCTAGGAA330.1751685333616434No Hit
CTGGATGACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTA330.1751685333616434No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT320.16986039598704814No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGA310.16455225861245287No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT300.15924412123785764No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG300.15924412123785764No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAA300.15924412123785764No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGGA270.14331970911407185No Hit
CTGGATGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA260.13801157173947662No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT260.13801157173947662No Hit
CTGGATGACTGGGATTAGATACCCCAGGAGACCTAGAGGAGCCTGTTCTA250.13270343436488136No Hit
CTGGATGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGGA240.1273952969902861No Hit
CTGGATGACTGGGATTAGATACCCCAAGAATCTGCTAGAGGAGCCTGTTC240.1273952969902861No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC240.1273952969902861No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG230.12208715961569085No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGGA210.11147088486650034No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT210.11147088486650034No Hit
CTGGATGACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAGG210.11147088486650034No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAA210.11147088486650034No Hit
CTGGATGACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG200.10616274749190507No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA200.10616274749190507No Hit
CTGGATGACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTT200.10616274749190507No Hit
CTGGATGACTGGGATTAGATACCCTATAAGAGGAGCCTGTTCTAGGAATC190.10085461011730984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG18400.0295.06
CTGGATT108.399036E-4295.09
CTGGATA108.399036E-4295.01
GACTGGA108.399036E-4295.07
ACTGGAT108.399036E-4295.08
CGACTGG108.399036E-4295.06
CTGGATG18650.0294.20911
ATGACTG18450.0294.200535
GACTGGG18450.0294.200537
TGGATGA18700.0293.422452
GGATGAC18600.0293.413973
CTGGGAT18450.0293.40119
ACTGGGA18500.0292.60818
GATGACT18750.0290.284
CTGGACG150.002827538196.666661
TGGACGA150.002827538196.666662
GGACGAC150.002827538196.666663
ACCCCTG950.059.00000420-24
CCCGACC650.059.00000420-24
ACCCCCT452.5465852E-1159.00000420-24
Produced by FastQC (version 0.11.5)