FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-78-4-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-78-4-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA308516.073568488511437No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG195210.170374615745324No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC17088.89907778877716No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA17078.893867555879748No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG7884.105663523159485No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA6563.4179127807012972No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA4252.2143489813994686No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG4152.162246652425363No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAG3952.0580419944771533No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3761.9590475694263536No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3611.8808940759651955No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3271.703746157453238No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2741.427603813890481No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGA2701.406762882300839No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTG2501.3025582243526286No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2211.1514614703277237No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2031.0576772781743344No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1570.8180065648934507No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTG1540.8023758662012193No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATT1460.7606940030219351No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA1330.6929609753555984No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1060.5522846871255145No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT1060.5522846871255145No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGATT1040.5418642213306935No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1030.536653988433283No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1030.536653988433283No Hit
GCCACAACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAG1000.5210232897410514No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA880.45850049497212525No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA860.4480800291773042No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGA820.42723909758766215No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG800.4168186317928411No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA780.4063981659980201No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGA770.40118793310060963No Hit
GCCACAACTGGGATTAGATACCCCTAGTAGTCCTAGAGGAGCCTGTTCTA750.39076746730578854No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA710.36992653571614653No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA690.3595060699213255No Hit
GCCACAACTGGGATTAGATACCCCGAGCACTAGAGGAGCCTGTTCTAGAT690.3595060699213255No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT690.3595060699213255No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC670.34908560412650447No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGATT670.34908560412650447No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT660.34387537122909395No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG650.33866513833168344No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA620.3230344396394519No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGA620.3230344396394519No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA610.31782420674204137No Hit
GCCACAACTGGGATTAGATACCCCACGAGGCCTAGAGGAGCCTGTTCTAG550.2865628093575783No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTG510.2657218777679362No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGA470.2448809461782942No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG470.2448809461782942No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA460.23967071328088366No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA430.2240400145886521No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGATTG430.2240400145886521No Hit
GCCACAACTGGGATTAGATACCCCAAGCCCACGAGACTAGAGGAGCCTGT420.2188297816912416No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG420.2188297816912416No Hit
GCCACAACTGGGATTAGATACCCCAACCGTCGTTCCACAATCGGCTCAAA410.21361954879383108No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA400.20840931589642056No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA400.20840931589642056No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT380.19798885010159956No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA360.1875683843067785No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA350.182358151409368No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGATTG340.1771479185119575No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGATT330.17193768561454698No Hit
GCCACAACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAG320.16672745271713646No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGATT320.16672745271713646No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC310.16151721981972594No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG290.1510967540249049No Hit
GCCACAACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGATTGA270.14067628823008388No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA270.14067628823008388No Hit
GCCACAACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAG270.14067628823008388No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA260.13546605533267336No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA240.12504558953785233No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGATT220.11462512374303131No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT220.11462512374303131No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT220.11462512374303131No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG220.11462512374303131No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGGGACTAGAGGAGCCTGT210.1094148908456208No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA200.10420465794821028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG18850.0295.06
AATGGGA108.4002607E-4295.06
ACAATGG108.4002607E-4295.04
TGGATTA157.1299346E-6294.999979
CTGGGAT18850.0294.21758
ACTGGGA18850.0294.21757
GCCACAA19200.0293.463531
CAACTGG19100.0293.455475
TGGGATT18950.0292.66499
CCACAAC19650.0285.241732
ACAACTG19700.0284.517764
CACAACT20000.0280.250033
CTGGATT202.2473741E-5221.258
ATGGGAT150.002827949196.666667
CAATGGG150.002827949196.666665
ACTGGAT255.4720564E-5177.07
AACTGGA403.557431E-4110.6256
TACCAAA650.002447536268.07693295
GGACCTG4050.059.000004105-109
CCAAAGG1250.059.000004100-104
Produced by FastQC (version 0.11.5)