FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-77-5-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-77-5-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18472
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC537429.092680814205284No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA16428.889129493287138No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT12076.534213945430922No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCT9615.202468601126029No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCT5372.9071026418362926No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5022.717626678215678No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4802.59852750108272No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTC3071.6619748809008228No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2911.575357297531399No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2691.4562581203984408No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2531.3696405370290168No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2471.337158943265483No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2461.3317453443048939No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTC2391.293850151580771No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTC2371.283022953659593No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2221.201818969250758No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCT1840.996102208748376No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1370.741663057600693No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1240.671286271113036No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1180.638804677349502No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1080.5846686877436119No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGT1010.546773495019489No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTC940.5088783022953659No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT900.48722390645300995No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAG890.481810307492421No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA860.465569510610654No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT840.45474231268947596No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCC790.42767431788653093No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT760.411433521004764No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC730.395192724122997No Hit
CTGGATGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC720.3897791251624079No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA700.37895192724122995No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT670.36271113035946295No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT670.36271113035946295No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA610.33022953659592896No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC590.319402338674751No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAA570.308575140753573No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT560.303161541792984No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT550.297747942832395No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT490.265266349068861No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC490.265266349068861No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCT480.25985275010827197No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA430.232784755305327No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC430.232784755305327No Hit
CTGGATGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAAG430.232784755305327No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCT420.22737115634473798No Hit
CTGGATGACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAAG420.22737115634473798No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAA410.22195755738414896No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAGT410.22195755738414896No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG410.22195755738414896No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA400.21654395842356No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCT400.21654395842356No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG390.21113035946297098No Hit
CTGGATGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAG370.20030316154179298No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG360.19488956258120396No Hit
CTGGATGACTGGGATTAGATACCCCCAGCACCTAGAGGAGCCTGTTCTAA340.18406236466002598No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCT330.178648765699437No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA330.178648765699437No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT320.17323516673884798No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA300.16240796881767No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGT280.151580770896492No Hit
CTGGATGACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC270.14616717193590298No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT270.14616717193590298No Hit
CTGGATGACTGGGATTAGATACCCCGACAACCTAGAGGAGCCTGTTCTAA260.140753572975314No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA260.140753572975314No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT260.140753572975314No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC260.140753572975314No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA250.13533997401472497No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA250.13533997401472497No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAAGT250.13533997401472497No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAAGT240.12992637505413598No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAAG240.12992637505413598No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT230.124512776093547No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG230.124512776093547No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT220.11909917713295798No Hit
CTGGATGACTGGGATTAGATACCCCCTGTCTCTTAAACCTAGAGGAGCCT220.11909917713295798No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACTAGACCTAGAGGAGCCT210.11368557817236899No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA200.10827197921178No Hit
CTGGATGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACA200.10827197921178No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT190.102858380251191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATT157.1275226E-6294.981059
TGGATGA18350.0294.981052
ACTGGGA18100.0294.981058
ACTGGAT157.1275226E-6294.981058
TGACTGG18250.0294.981026
CTGGATG18400.0294.981021
GACTGGG18150.0294.981027
GGATGAC18350.0294.177283
GATGACT18300.0294.175084
CTGGGAT18150.0294.168439
ATGACTG18350.0293.37355
AATCAGC150.0028276548196.65404295
GACTGGA255.4702155E-5176.988627
ACCCCTC351.3855242E-859.15652520-24
ACCCCGG257.656143E-659.15652520-24
CCCGACC501.8189894E-1259.15652520-24
CCCGGTA201.8145861E-459.1565220-24
CCAGTAG405.9299055E-1059.1565220-24
CCGCCAA150.004322147559.1565220-24
CCCATCT150.004322147559.1565220-24
Produced by FastQC (version 0.11.5)