FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-76-5-5-7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-76-5-5-7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18311
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC558430.495330675550214No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA13477.356233957730327No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT11676.3732182840915295No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTG9355.106220304734859No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5362.92720222816886No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCT4802.6213751297034573No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4792.615913931516575No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCC4512.463000382283873No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT4482.4466167877232268No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2581.408989132215608No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCT2401.3106875648517287No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2231.217847195674731No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2191.1960024029272023No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1871.0212440609469717No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTC1810.9884768718256786No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCC1790.9775544754519142No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1610.8792529080880345No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1380.7536453497897438No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1170.6389601878652176No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1140.622576593304571No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT1100.6007318005570422No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCT1040.567964611435749No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT940.513352629566927No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT870.47512424225875155No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT860.4696630440718694No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC840.45874064769810496No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT830.4532794495112228No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA780.42597345857681174No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAT780.42597345857681174No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT770.42051226038992956No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT750.40958986401616515No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT740.404128665829283No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT740.404128665829283No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT600.32767189121293216No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT530.2894435039047567No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT520.2839823057178745No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAT520.2839823057178745No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA470.2566763147834635No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCT440.24029272022281686No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC430.2348315220359347No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT420.22937032384905248No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCCT400.21844792747528807No Hit
CTGGATGACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTATT400.21844792747528807No Hit
CTGGATGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCC390.21298672928840587No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATT380.2075255311015237No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCC360.19660313472775925No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG360.19660313472775925No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAT350.19114193654087708No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC340.18568073835399487No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTC330.18021954016711267No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA320.17475834198023046No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT320.17475834198023046No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT310.16929714379334826No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTC300.16383594560646608No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATTC300.16383594560646608No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTC290.15837474741958385No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA290.15837474741958385No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA290.15837474741958385No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT280.15291354923270167No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCC270.14745235104581944No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT260.14199115285893724No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG260.14199115285893724No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT250.13652995467205506No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTC250.13652995467205506No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT240.13106875648517283No Hit
CTGGATGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA240.13106875648517283No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA230.12560755829829065No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCC230.12560755829829065No Hit
CTGGATGACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTATTCCT220.12014636011140843No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC220.12014636011140843No Hit
CTGGATGACTGGGATTAGATACCCCACACGGACCTAGAGGAGCCTGTTCT210.11468516192452624No Hit
CTGGATGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC200.10922396373764404No Hit
CTGGATGACTGGGATTAGATACCCCACCAAACCTAGAGGAGCCTGTTCTA190.10376276555076185No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTATTC190.10376276555076185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC18150.0295.03
ATGACGG157.124743E-6295.05
GACGGGA108.397208E-4295.07
GATGACG157.124743E-6295.04
CTGGATT108.397208E-4295.09
CGGGATT108.397208E-4295.09
ACGGGAT108.397208E-4295.08
TGGATGA18150.0295.02
TGACGGG108.397208E-4295.06
CTGGATG18300.0294.1941
GACTGGG17750.0294.169047
CTGGGAT17650.0294.16439
ACTGGGA17650.0294.16438
GATGACT18100.0293.370184
TGACTGG17900.0293.351966
ATGACTG18000.0292.541665
TGACTTG200.0066839894147.56
TAATCCA200.0066839894147.5295
GACTGGA200.0066839894147.57
TTACCGA700.059.00000455-59
Produced by FastQC (version 0.11.5)