FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-75-4-8-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-75-4-8-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18530
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA323717.468969239071775No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT224812.131678359417162No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG13117.075013491635186No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT9625.191581219643822No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA7574.085267134376687No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA6393.448461953588775No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCA3932.1208850512682136No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3451.8618456556934702No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCAC3161.7053426875337292No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC3151.6999460334592553No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT3011.6243928764166218No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2921.5758229897463574No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT2451.3221802482460874No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2271.2250404749055586No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2231.2034538586076633No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1540.8310847274689692No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1480.7987048030221263No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1470.7933081489476526No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAG1440.7771181867242309No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATA1350.7285483000539665No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAA1340.7231516459794928No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATA1260.6799784133837021No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC1220.6583917970858069No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1060.5720453318942256No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT1030.5558553696708041No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT1010.5450620615218564No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1000.5396654074473827No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA950.5126821370750134No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATA840.4533189422558014No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA830.44792228818132757No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGC780.4209390178089584No Hit
CAACACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATA750.404749055585537No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATA740.39935240151106316No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC730.39395574743658934No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA690.372369131138694No Hit
CAACACACTGGGATTAGATACCCCGACGTGCTAGAGGAGCCTGTTCTATA650.3507825148407987No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA640.3453858607663249No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAT620.3345925526173772No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA580.3130059363194819No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCA570.3076092822450081No Hit
CAACACACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTATAA560.30221262817053424No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAT540.2914193200215866No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA540.2914193200215866No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA530.2860226659471128No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG520.28062601187263897No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA520.28062601187263897No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT510.27522935779816515No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTATA510.27522935779816515No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA500.26983270372369134No Hit
CAACACACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTATAAGC500.26983270372369134No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAAG490.26443604964921746No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA480.2590393955747437No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCA470.25364274150026983No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAG470.25364274150026983No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATAAG450.24284943335132217No Hit
CAACACACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTATAAG440.23745277927684835No Hit
CAACACACTGGGATTAGATACCCCGATCCCACGAGACCTAGAGGAGCCTG430.23205612520237454No Hit
CAACACACTGGGATTAGATACCCCTAAGAGGAGCCTGTTCTATAAGCACT410.2212628170534269No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATA390.2104695089044792No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA380.20507285483000542No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA370.19967620075553158No Hit
CAACACACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAAG360.19427954668105774No Hit
CAACACACTGGGATTAGATACCCCACTAGTCCTAGAGGAGCCTGTTCTAT360.19427954668105774No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAAG350.18888289260658392No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC350.18888289260658392No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATAA340.1834862385321101No Hit
CAACACACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTAT340.1834862385321101No Hit
CAACACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG320.17269293038316244No Hit
CAACACACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA310.1672962763086886No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAT310.1672962763086886No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAGC300.16189962223421478No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCA300.16189962223421478No Hit
CAACACACTGGGATTAGATACCCCCACGGACCTAGAGGAGCCTGTTCTAT290.15650296815974096No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATAAGC290.15650296815974096No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATAAG280.15110631408526712No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT280.15110631408526712No Hit
CAACACACTGGGATTAGATACCCCCGAGATCTAGAGGAGCCTGTTCTATA260.14031300593631948No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT260.14031300593631948No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA250.13491635186184567No Hit
CAACACACTGGGATTAGATACCCCGAGACCACGAGACCTAGAGGAGCCTG250.13491635186184567No Hit
CAACACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAAG230.124123043712898No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG230.124123043712898No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA200.10793308148947653No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATA190.10253642741500271No Hit
CAACACACTGGGATTAGATACCCCGAGCCCGACTAGAGGAGCCTGTTCTA190.10253642741500271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG255.11136E-10295.06
ACGCTGG255.11136E-10295.05
CACTGGG17750.0295.06
CGGGATT157.1260056E-6295.08
ACGGGAT157.1260056E-6295.07
TGGGATA108.397951E-4295.09
CTGGGAT18000.0294.180578
ACACTGG17900.0294.175965
ACTGGGA17750.0294.1697
TGGGATT17950.0293.356549
CACACTG18200.0290.137364
ACACACT19250.0275.844183
GCTGGGA301.5206751E-9245.833347
CAACACA22750.0236.000021
AACACAC22700.0235.870042
ACACGGG202.2461387E-5221.255
CACACGG202.2461387E-5221.254
CACGGGA202.2461387E-5221.256
TAGGATT150.0028271729196.666679
CACTGGA150.0028271729196.666676
Produced by FastQC (version 0.11.5)