FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-74-4-1-3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-74-4-1-3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18302
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC287815.72505737077915No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA255113.93836739154191No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCT189710.364987433067425No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCT9395.130586821112447No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6923.7810075401595458No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT6893.764615888973882No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCC4152.2675117473500164No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3932.147306305321823No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2871.5681346300950714No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTC2751.5025680253524205No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2661.4533930717954322No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2651.4479291880668779No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTC2641.4424653043383238No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2521.3768986995956725No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCT2521.3768986995956725No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTC2151.174735001639165No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2041.1146322806250684No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1991.087312861982297No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1520.8305103267402469No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1240.6775215823407278No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1210.661129931155065No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1200.6556660474265108No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA1140.6228827450551853No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1070.5846355589553054No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCT950.5190689542126543No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAG930.5081411867555458No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT770.4207190470986778No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA750.40979127964156925No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTC720.3933996284559064No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT640.3496885586274724No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC640.3496885586274724No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT630.3442246748989181No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCT630.3442246748989181No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA610.3332969074418097No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT600.3278330237132554No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAAGT590.3223691399847011No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA570.31144137252759263No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAG560.30597748879903836No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTC540.29504972134192986No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT540.29504972134192986No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGT520.2841219538848213No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA510.2786580701562671No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCT500.2731941864277128No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCT500.2731941864277128No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCT490.26773030269915854No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAG490.26773030269915854No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCT490.26773030269915854No Hit
ATCGGTTACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAAGTCT490.26773030269915854No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC460.25133865151349577No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA450.24587476778494152No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT420.22948311659927878No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGCTAGAGGAGCCTGTTCTAAG420.22948311659927878No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTC410.22401923287072453No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTA400.21855534914217026No Hit
ATCGGTTACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAG370.20216369795650746No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT350.19123593049939897No Hit
ATCGGTTACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAAGTC340.18577204677084472No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA310.16938039558518195No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG310.16938039558518195No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCACGCCGTAAACGATGTC310.16938039558518195No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT300.1639165118566277No Hit
ATCGGTTACTGGGATTAGATACCCACGCAACCTAGAGGAGCCTGTTCTAA280.15298874439951918No Hit
ATCGGTTACTGGGATTAGATACCCTGTGTATAGAGGAGCCTGTTCTAAGT280.15298874439951918No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAGT280.15298874439951918No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTC270.14752486067096493No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT270.14752486067096493No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA260.14206097694241065No Hit
ATCGGTTACTGGGATTAGATACCCCCCTAAGCTAGAGGAGCCTGTTCTAA260.14206097694241065No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTAAGT250.1365970932138564No Hit
ATCGGTTACTGGGATTAGATACCCCGACCCAACCTAGAGGAGCCTGTTCT250.1365970932138564No Hit
ATCGGTTACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA240.13113320948530216No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAAGT230.12566932575674788No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCCACGAGACCTAGAGGAGCCT220.12020544202819364No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT210.11474155829963939No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAAGTC210.11474155829963939No Hit
ATCGGTTACTGGGATTAGATACCCCACACGGACCTAGAGGAGCCTGTTCT200.10927767457108513No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGATACCTAGAGGAGCCT190.10381379084253087No Hit
ATCGGTTACTGGGATTAGATACCCCGACGACCTAGAGGAGCCTGTTCTAA190.10381379084253087No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTCTAGAGGAGCCTGTTCTAAG190.10381379084253087No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCT190.10381379084253087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG108.397208E-4295.06
ATGGGAT108.397208E-4295.08
CTGGGTT108.397208E-4295.09
TTACTGG17850.0295.06
TCGGTTA18100.0295.02
TCGGTAC206.021037E-8295.02
GGTTATG108.397208E-4295.04
ATCGGTA206.021037E-8295.01
CTGGATT206.021037E-8295.09
CGGTTAT108.397208E-4295.03
ACTGGGT108.397208E-4295.08
GGTACTG206.021037E-8295.04
ATCGGTT18150.0294.187351
CGGTTAC18050.0294.182833
GGTTACT18000.0294.180544
GTTACTG17900.0294.1765
TACTGGG17850.0291.694677
ACTGGGA17800.0290.856728
CTGGGAT17750.0290.84519
GTACTGG251.8323772E-7236.05
Produced by FastQC (version 0.11.5)