FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-73-4-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-73-4-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17695
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG357220.18649335970613No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAT215112.155976264481492No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTCT12917.295846284261091No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG7504.238485447866629No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGAT7204.068946029951963No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAGGA4352.458321559762645No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATTC4072.3000847697089575No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAT3762.1248940378638035No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC3411.927098050296694No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAGGA3251.8366770274088724No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2911.644532353772252No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2881.6275784119807857No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAATA2841.6049731562588303No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAT2771.5654139587454083No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2401.3563153433173212No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGGAT2171.226335122916078No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGATT1841.0398417632099464No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1610.909861542808703No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA1490.8420457756428369No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1490.8420457756428369No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA1420.8024865781294152No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA1250.7064142413111049No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAATAGGAT1190.6725063577281718No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAATA1140.6442497880757276No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAATA1090.6159932184232834No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAATAG990.559480079118395No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAATAG900.5086182537439954No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA860.48601299802204007No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG820.46340774230008475No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG800.4521051144391071No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA800.4521051144391071No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAATAGG770.4351511726476406No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG750.42384854478666295No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAATA700.3955919751342187No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAT690.3899406612037299No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGGAT670.3786380333427522No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCTAGAGGAGCCTGTTCTAATAG660.37298671941226336No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG640.36168409155128567No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAATAG620.350381463690308No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAGGA600.3390788358293303No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG570.3221248940378638No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAATA520.2938683243854196No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA520.2938683243854196No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG510.2882170104549308No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG460.2599604408024866No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT440.2486578129415089No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAA440.2486578129415089No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT420.23735518508053122No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG420.23735518508053122No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAATAGGA410.23170387115004237No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGAT410.23170387115004237No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAAT400.22605255721955356No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG400.22605255721955356No Hit
AAGCGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAATA400.22605255721955356No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAATAGGAT380.21474992935857587No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG360.2034473014975982No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG360.2034473014975982No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAAT350.19779598756710934No Hit
AAGCGACTGGGATTAGATACCCCCCTGTCTCCCTAGAGCTAGAGGAGCCT340.1921446736366205No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC340.1921446736366205No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAATA320.18084204577564283No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT320.18084204577564283No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT310.175190731845154No Hit
AAGCGACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTTC310.175190731845154No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAATAGGA300.16953941791466515No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG300.16953941791466515No Hit
AAGCGACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTAAT300.16953941791466515No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAATAGGAT300.16953941791466515No Hit
AAGCGACTGGGATTAGATACCCCTGTCTCTTATACACATGGCTAGAGGAG290.1638881039841763No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG280.1582367900536875No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC280.1582367900536875No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAATAGG270.15258547612319864No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGATT260.1469341621927098No Hit
AAGCGACTGGGATTAGATACCCCGCCCGAGACCTAGAGGAGCCTGTTCTA260.1469341621927098No Hit
AAGCGACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAATAGG250.14128284826222096No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAATAGG250.14128284826222096No Hit
AAGCGACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAATAGGA250.14128284826222096No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAATA240.13563153433173214No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC240.13563153433173214No Hit
AAGCGACTGGGATTAGATACCCCTGAGCTGCCGACTAGAGGAGCCTGTTC190.10737496467928793No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG190.10737496467928793No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT190.10737496467928793No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA180.1017236507487991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT17500.0295.07
GACTGGG17550.0295.05
AAGCGAC17650.0295.01
TGGGATT17300.0295.08
TGGGATA206.015034E-8295.08
ACTGGGA17500.0295.06
CGACTGG17600.0295.04
AGCGACT17650.0294.16432
GGGATTA17300.0294.14749
GCGACTG17700.0293.333343
GGGATAG251.8305218E-7236.09
CAGCCCT150.00436713859.00000430-34
GGAGATC303.3070864E-759.00000425-29
GTCACTT150.00436713859.000004155-159
TTGCAAA150.00436713859.000004220-224
CCTACGA150.00436713859.00000420-24
ATCTCCA150.00436713859.000004170-174
AGAAGCC150.00436713859.000004135-139
CCCGGTA150.00436713859.00000420-24
TATGCTC150.00436713859.00000425-29
Produced by FastQC (version 0.11.5)