FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-72-5-8-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-72-5-8-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17648
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA595933.76586582048957No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA11616.578649138712602No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG7644.329102447869447No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC7304.136446056210335No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA5403.059836808703536No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA5262.980507706255666No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA5072.8728467815049865No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG3762.1305530371713512No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2721.5412511332728922No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2471.3995920217588396No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG2461.3939256572982774No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1821.0312783318223029No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1791.0142792384406165No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGA1781.0086128739800544No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA1670.9462828649138713No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1580.8952855847688124No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1580.8952855847688124No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTG1300.7366273798730735No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG1300.7366273798730735No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1290.7309610154125114No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTG1150.651631912964642No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGAT1120.6346328195829556No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAG1090.6176337262012692No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1050.5949682683590208No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC920.5213055303717135No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA920.5213055303717135No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA830.4703082502266546No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG790.4476427923844062No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA780.4419764279238441No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA760.43064369900271987No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA720.40797824116047143No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC690.3909791477787851No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA670.37964641885766093No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGATT660.3739800543970988No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA610.3456482320942883No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA610.3456482320942883No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC560.3173164097914778No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA520.29465095194922936No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT470.2663191296464189No Hit
CAACACACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTAGATTG470.2663191296464189No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG460.2606527651858567No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG410.23232094288304625No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA400.22665457842248413No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATT400.22665457842248413No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTG390.22098821396192206No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGA370.20965548504079784No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG360.20398912058023572No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT340.19265639165911153No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGA330.1869900271985494No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA320.1813236627379873No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG310.1756572982774252No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTGA310.1756572982774252No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTGA290.164324569356301No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTGA280.1586582048957389No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGA280.1586582048957389No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCTG270.1529918404351768No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGA270.1529918404351768No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA270.1529918404351768No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA260.14732547597461468No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG250.14165911151405258No Hit
CAACACACTGGGATTAGATACCCCCGCAATCTAGAGGAGCCTGTTCTAGA250.14165911151405258No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA250.14165911151405258No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA240.1359927470534905No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCT240.1359927470534905No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA230.13032638259292836No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT230.13032638259292836No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT230.13032638259292836No Hit
CAACACACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCTA220.12466001813236627No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGATTG210.11899365367180417No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGAT210.11899365367180417No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCATAAACATTGGTA200.11332728921124206No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGATTG190.10766092475067997No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA190.10766092475067997No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCTCTAGAGGAGCCTGTTCTA190.10766092475067997No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT190.10766092475067997No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGATTG180.10199456029011786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG157.120094E-6295.06
ACGCTGG157.120094E-6295.05
GCTGGGA157.120094E-6295.07
ACACTGG17200.0295.05
CACTGGG17150.0295.06
TGGGATT17300.0293.29489
ACTGGGA17150.0293.279887
CACACTG17400.0292.456884
CTGGGAT17300.0292.44228
ACACACT18350.0277.316073
AACACAC21200.0240.035372
CAACACA21650.0236.408781
CACGCTG202.244278E-5221.254
ACACGCT255.4645323E-5177.03
TATAATA754.325765E-578.666664295
AACACGC600.001779322273.752
CAACACG650.002444218868.076931
CAGCCCT452.5465852E-1159.00000430-34
AATCTTG650.059.00000440-44
GATACTT650.059.00000445-49
Produced by FastQC (version 0.11.5)