FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-71-6-5-8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-71-6-5-8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17033
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA878951.59983561322139No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA6473.7985087770797863No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA5683.334703223155052No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA4712.765220454412024No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA4042.3718663770328186No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA3442.0196089943051723No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA3111.8258674338049667No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA3081.8082545646685846No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCAC2851.6732225679563202No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA2661.5616743967592321No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA2441.432513356425762No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA2141.2563846650619386No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA2091.227029883167968No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA2011.180062232137615No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1670.9804497152586156No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA1110.6516761580461456No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA1010.5929665942582046No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT980.5753537251218224No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC790.4638055539247343No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCA770.45206364116714615No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAG630.36987025186402867No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA600.35225738272764634No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC570.334644513591264No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA540.3170316444548817No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG490.2876768625609112No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA410.24070921153055833No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA400.23483825515176424No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA390.2289672987729701No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG390.2289672987729701No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA380.223096342394176No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA370.2172253860153819No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA360.21135442963658782No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA350.20548347325779368No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA320.18787060412141138No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAA320.18787060412141138No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC300.17612869136382317No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGC250.14677390946985264No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACCATCCAATC250.14677390946985264No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA250.14677390946985264No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA240.14090295309105852No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAA230.13503199671226443No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAG230.13503199671226443No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC230.13503199671226443No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAA230.13503199671226443No Hit
TGATTGAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC210.12329008395467621No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG210.12329008395467621No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA210.12329008395467621No Hit
TGATTGACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC190.111548171197088No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA190.111548171197088No Hit
TGATTGACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATAA190.111548171197088No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA190.111548171197088No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC180.10567721481829391No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA180.10567721481829391No Hit
TGATTGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA180.10567721481829391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGA16950.0294.958831
GACACTG16850.0294.958836
TTGACAC16900.0294.958834
GATTGAC16950.0294.088752
ACACTGG16850.0294.08367
ACTGGGA16850.0294.08369
CACTGGG16800.0294.080968
ATTGACA17000.0293.223753
TGACACT16950.0293.218665
TACTGAA150.0028263372196.6392295
CTCACCA200.0066826125147.4794295
CAGCCCT405.9117156E-1059.16578335-39
CCTAAAG201.8123619E-459.16578335-39
TAGCCGT1100.059.16578335-39
GGCCCTA550.059.16578335-39
CCGTAAA1650.059.16578335-39
CGCCGTA550.059.16578335-39
GCCCTAA12550.059.1657835-39
CCCGCTA303.2420212E-759.1657820-24
CCCCGTC150.00431829659.1657820-24
Produced by FastQC (version 0.11.5)