FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-70-5-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-70-5-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16853
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC506530.053996321129766No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA14208.425799560909036No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT9755.785320121046698No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACT9725.767519136058862No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGC5973.542396012579363No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4052.403132973357859No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3822.266658755117783No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAG2801.6614252655313595No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2501.4834154156529995No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2391.4181451373642675No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA2351.3944104907138195No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2231.3232065507624755No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAG2081.2342016258232955No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCAC1901.1273957158962795No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGC1460.8663146027413517No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1340.7951106627900076No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1280.7595086928143358No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCA1270.7535750311517238No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1240.7357740461638878No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAA1140.6764374295377678No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1110.6586364445499318No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1100.6527027828873198No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1100.6527027828873198No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAG990.5874325045985878No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT910.5399632112976919No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC820.48656025633418387No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT810.48062659467157187No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT810.48062659467157187No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAG710.4212899780454519No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT640.3797543464071679No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA620.3678870230819439No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT610.3619533614193319No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT600.3560196997567199No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATA570.3382187147688839No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA560.3322850531062719No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT560.3322850531062719No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT550.3263513914436599No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGC540.3204177297810479No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA470.2788820981427639No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGC450.26701477481753993No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT450.26701477481753993No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT440.2610811131549279No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTATA440.2610811131549279No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG440.2610811131549279No Hit
CTGGATGACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTATAAG420.24921378982970388No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT410.24328012816709194No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGC400.23734646650447994No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC400.23734646650447994No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATA400.23734646650447994No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT400.23734646650447994No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT380.2254791431792559No Hit
CTGGATGACTGGGATTAGATACCCCTGCTGACTAGAGGAGCCTGTTCTAT350.20767815819141994No Hit
CTGGATGACTGGGATTAGATACCCCGCCCGACCTAGAGGAGCCTGTTCTA340.20174449652880794No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA310.18394351154097194No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA310.18394351154097194No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTTCTAGAGGAGCCTGTTCTA310.18394351154097194No Hit
CTGGATGACTGGGATTAGATACCCCACGAGAACTAGAGGAGCCTGTTCTA300.17800984987835994No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT300.17800984987835994No Hit
CTGGATGACTGGGATTAGATACCCCACTATACACATTACGCTAGAGGAGC280.16614252655313594No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGC270.16020886489052394No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT250.14834154156529994No Hit
CTGGATGACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCT250.14834154156529994No Hit
CTGGATGACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA250.14834154156529994No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT250.14834154156529994No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG250.14834154156529994No Hit
CTGGATGACTGGGATTAGATACCCCTGACTGCCAACTAGAGGAGCCTGTT240.14240787990268794No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGC240.14240787990268794No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT230.13647421824007597No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC230.13647421824007597No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.13054055657746394No Hit
CTGGATGACTGGGATTAGATACCCCTGACACCTAGAGGAGCCTGTTCTAT220.13054055657746394No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG220.13054055657746394No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA210.12460689491485194No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTA210.12460689491485194No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAAC190.11273957158962795No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGGACCTAGAGGAGC180.10680590992701597No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC170.10087224826440397No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGC170.10087224826440397No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGGCCTAGAGGAGCCT170.10087224826440397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG16450.0295.06
CTGGATT157.114686E-6295.09
CTGGATG16700.0295.01
TGGATGA16650.0294.999972
GGATGAC16650.0294.11413
GATGACT16550.0294.108734
GACTGGG16400.0293.20127
ACTGGGA16400.0293.20128
ATGACTG16600.0292.334355
CTGGGAT16450.0292.310039
GACTGGA202.2425767E-5221.257
ACTGGAT202.2425767E-5221.258
CTGGAGA150.0028249354196.666671
AGACTGG200.006679301147.55
AACGCTT452.5465852E-1159.00000490-94
ATCCCAC150.004366450559.000004175-179
AAACTTC452.5465852E-1159.00000440-44
CTTCAAC452.5465852E-1159.00000440-44
TCCCACG150.004366450559.000004175-179
AACCGCT600.059.00000465-69
Produced by FastQC (version 0.11.5)