FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-7-4-5-7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-7-4-5-7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11269
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC208618.510959268790486No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA131511.669180938858817No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTG9478.403585056349277No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5334.729789688526044No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCT4834.28609459579377No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCC4243.762534386369687No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3483.0881178454166296No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCT2091.8546454876209069No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2061.8280237820569705No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCC1831.6239240394001244No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1771.5706806282722512No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTC1611.4286981985979235No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1361.2068506522317863No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1351.1979767503771408No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1171.038246516993522No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCT1120.9938770077202946No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC940.8341467743366758No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT900.7986511669180938No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA860.7631555594995119No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT860.7631555594995119No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAT830.7365338539355755No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT780.692164344662348No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT760.674416540953057No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT680.6034253261158932No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT670.5945514242612476No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAT520.46144289644156533No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC510.45256899458691985No Hit
CTGGATGACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTATT510.45256899458691985No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATT500.4436950927322744No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT490.4348211908776289No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCT460.40819948531369243No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT450.3993255834590469No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAT440.39045168160440147No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCCT410.363829976040465No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT400.3549560741858195No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA390.346082172331174No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTC370.32833436862188303No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT370.32833436862188303No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCC370.32833436862188303No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATTC370.32833436862188303No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT340.3017126630579466No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC320.2839648593486556No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC310.27509095749401014No Hit
CTGGATGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA300.2662170556393646No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTC300.2662170556393646No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT280.24846925193007366No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT280.24846925193007366No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT270.23959535007542815No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA260.23072144822078267No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTC250.2218475463661372No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT250.2218475463661372No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTATTC250.2218475463661372No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCC240.21297364451149167No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC230.20409974265684622No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATTC220.19522584080220073No Hit
CTGGATGACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGG210.18635193894755525No Hit
CTGGATGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC200.17747803709290974No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA200.17747803709290974No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTC200.17747803709290974No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCT180.15973023338361877No Hit
CTGGATGACTGGGATTAGATACCCCACCAAACCTAGAGGAGCCTGTTCTA170.1508563315289733No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCC170.1508563315289733No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT170.1508563315289733No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA170.1508563315289733No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTTCCTCTGTCTCTTATA170.1508563315289733No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT160.1419824296743278No Hit
CTGGATGACTGGGATTAGATACCCCAGATAGAGGAGCCTGTTCTATTCCT160.1419824296743278No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTCCTCTGTCTCTTATACA160.1419824296743278No Hit
CTGGATGACTGGGATTAGATACCCCACACGGACCTAGAGGAGCCTGTTCT150.1331085278196823No Hit
CTGGATGACTGGGATTAGATACCCCTGAAGACCTAGAGGAGCCTGTTCTA150.1331085278196823No Hit
CTGGATGACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTATTCCT150.1331085278196823No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT150.1331085278196823No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT150.1331085278196823No Hit
CTGGATGACTGGGATTAGATACCCCCGACCGACCTAGAGGAGCCTGTTCT130.11536072411039133No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT130.11536072411039133No Hit
CTGGATGACTGGGATTAGATACCCCAGTCTGTCTAGAGGAGCCTGTTCTA130.11536072411039133No Hit
CTGGATGACTGGGATTAGATACCCCGACGACCTAGAGGAGCCTGTTCTAT130.11536072411039133No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT120.10648682225574584No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT120.10648682225574584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG11050.0295.06
CTGGATT157.0517035E-6295.09
CTGGATG11250.0295.01
CGGGATT108.3541445E-4295.09
ACGGGAT108.3541445E-4295.08
GACTGGG10900.0295.07
ACTGGGA10900.0295.08
TGGATGA11250.0293.688872
GGATGAC11200.0293.683043
CTGGGAT10900.0293.64689
ATGACTG11150.0292.354255
GATGACT11150.0292.354254
GACTGGA202.2227601E-5221.257
GACGGGA150.0028124624196.666677
TGACGGG150.0028124624196.666676
ATGACGG200.0066498853147.55
GATGACG200.0066498853147.54
ACTGGAT301.11929636E-4147.58
GGAGATC150.00435891359.00000425-29
CGTCAGT150.00435891359.000004200-204
Produced by FastQC (version 0.11.5)