FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-69-4-8-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-69-4-8-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17408
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA247014.188878676470587No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG218912.574678308823529No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT14858.530560661764707No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG10636.106387867647059No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG8024.607077205882353No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC5243.010110294117647No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA4692.6941636029411766No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGC4302.4701286764705883No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG3992.292049632352941No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTG2741.5739889705882353No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGG2711.5567555147058825No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT2591.4878216911764706No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG2541.4590992647058825No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2431.3959099264705883No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2231.2810202205882353No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1881.0799632352941175No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1761.0110294117647058No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA1570.9018841911764706No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTG1510.8674172794117647No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG1470.844439338235294No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGT1440.8272058823529412No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGT1280.7352941176470588No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGA1250.7180606617647058No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTG970.5572150735294118No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA950.5457261029411764No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGACG860.49402573529411764No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGACGTG800.4595588235294118No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAG800.4595588235294118No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGT790.45381433823529416No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA770.4423253676470588No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGAC760.4365808823529412No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC750.43083639705882354No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTG730.4193474264705882No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACGT730.4193474264705882No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG720.4136029411764706No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT670.38488051470588236No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGA660.3791360294117647No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA650.3733915441176471No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT630.3619025735294118No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG620.3561580882352941No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA620.3561580882352941No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA580.33318014705882354No Hit
CAACACACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCTA570.3274356617647059No Hit
CAACACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT550.3159466911764706No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA520.29871323529411764No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTG510.29296875No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGA510.29296875No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT480.2757352941176471No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG480.2757352941176471No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA450.2585018382352941No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTG440.25275735294117646No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG420.24126838235294118No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA420.24126838235294118No Hit
CAACACACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTAGAC410.2355238970588235No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG400.2297794117647059No Hit
CAACACACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCT400.2297794117647059No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA380.2182904411764706No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA380.2182904411764706No Hit
CAACACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT360.2068014705882353No Hit
CAACACACTGGGATTAGATACCCGAGTAGTCCTAGAGGAGCCTGTTCTAG360.2068014705882353No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGACG360.2068014705882353No Hit
CAACACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAG340.1953125No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGAC340.1953125No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA330.18956801470588236No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA320.1838235294117647No Hit
CAACACACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTAGAC310.17807904411764705No Hit
CAACACACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAGAC270.15510110294117646No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGA270.15510110294117646No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGGACCTAGAGGAGCC270.15510110294117646No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA240.13786764705882354No Hit
CAACACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAG230.1321231617647059No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA220.12637867647058823No Hit
CAACACACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTAGACGT220.12637867647058823No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT220.12637867647058823No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA220.12637867647058823No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG210.12063419117647059No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTGG200.11488970588235295No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGACG200.11488970588235295No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGAC190.1091452205882353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG17100.0295.06
CCCTGGG108.393649E-4295.06
ACCCTGG108.393649E-4295.05
TGGGATA157.118697E-6295.09
ACTGGGA17200.0293.284887
CTGGGAT17350.0292.449558
TGGGATT17200.0292.427349
ACACTGG17300.0291.58965
CACACTG17350.0290.749274
ACACACT18500.0272.675663
CAACACA21050.0241.045151
AACACAC22300.0226.872212
CACCCTG200.006681171147.54
CCTGGGA200.006681171147.57
GCCCTAA3600.059.00000435-39
AAACTCC150.004366929659.00000440-44
CTGAGCA303.3065407E-759.00000450-54
TGGTAGC150.004366929659.00000445-49
CGCCTGG150.004366929659.00000470-74
CCCGGTA600.059.00000420-24
Produced by FastQC (version 0.11.5)