FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-68-4-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-68-4-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16786
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG272616.23972357917312No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACA201912.02788037650423No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGTC10776.416061003216966No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATCT9815.844155844155844No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG5543.3003693554152265No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGATC5283.1454783748361725No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACA3762.239961872989396No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTGT3051.8169903491004409No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACA3031.8050756582866676No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCTG3011.793160967472894No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAGA2871.7097581317764805No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2761.6442273323007268No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGATC2381.4178482068390326No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC2381.4178482068390326No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2111.2569998808530918No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACAG2061.2272131538186584No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATCT2031.2093411175979984No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGATCT1821.084236864053378No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC1811.078279518646491No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC1570.9353032288812105No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1480.8816871202192303No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC1420.8459430477779102No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGATC1220.7267961396401764No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACAG1160.6910520671988561No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1150.6850947217919695No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1150.6850947217919695No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1080.6433933039437627No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTACAGAT1010.6016918860955558No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATCT920.5480757774335756No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGATCT890.5302037412129156No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGATC810.482544977957822No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACA800.47658763255093534No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT770.45871559633027525No Hit
AAGCGACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTAC730.4348862147027285No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACAG710.4229715238889551No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT700.4170141784820684No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGAT680.40509948766829496No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACAGATCTG670.39914214226140826No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACAG650.38722745144763493No Hit
AAGCGACTGGGATTAGATACCCCTACGAGACCTAGAGGAGCCTGTTCTAC630.37531276063386154No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG630.37531276063386154No Hit
AAGCGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTACAGAT620.36935541522697485No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG580.3455260335994281No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCCCTAGAGGAGCCTGTTCTACA570.3395686881925414No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT560.3336113427856547No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTACA550.327653997378768No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAGAT550.327653997378768No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGATCT550.327653997378768No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG530.31573930656499466No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACAGATC500.2978672703443346No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG490.2919099249374479No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTACA490.2919099249374479No Hit
AAGCGACTGGGATTAGATACCCCCGTGCTAGAGGAGCCTGTTCTACAGAT460.2740378887167878No Hit
AAGCGACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTACA460.2740378887167878No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACAG460.2740378887167878No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACAGAT430.2561658524961277No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG420.25020850708924103No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACAG420.25020850708924103No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTACAGA380.22637912546169428No Hit
AAGCGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTACAG380.22637912546169428No Hit
AAGCGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTACAG370.2204217800548076No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTACA370.2204217800548076No Hit
AAGCGACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACAGAT360.2144644346479209No Hit
AAGCGACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTACAGA350.2085070892410342No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACA340.20254974383414748No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC330.19659239842726078No Hit
AAGCGACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTACAG320.19063505302037412No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACAG300.17872036220660076No Hit
AAGCGACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTACA300.17872036220660076No Hit
AAGCGACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACAGATC290.17276301679971404No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG290.17276301679971404No Hit
AAGCGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTACAG280.16680567139282734No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACAGA280.16680567139282734No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACAGAT280.16680567139282734No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATCT270.16084832598594068No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCAGACCCTAGAGGAGCCTG270.16084832598594068No Hit
AAGCGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA270.16084832598594068No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG270.16084832598594068No Hit
AAGCGACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTACAGATC260.15489098057905398No Hit
AAGCGACTGGGATTAGATACCCCGTCCTAGAGGAGCCTGTTCTACAGATC260.15489098057905398No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC250.1489336351721673No Hit
AAGCGACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACAGAT250.1489336351721673No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG250.1489336351721673No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG240.1429762897652806No Hit
AAGCGACTGGGATTAGATACCCTGTCTAGAGGAGCCTGTTCTACAGATCT230.1370189443583939No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT210.12510425354462051No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGATCT200.11914690813773383No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTACA190.11318956273084714No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG190.11318956273084714No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT180.10723221732396045No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA180.10723221732396045No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG180.10723221732396045No Hit
AAGCGACTGGGATTAGATACCCTCGAGCCCACGAGACCTAGAGGAGCCTG170.10127487191707374No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACAGAT170.10127487191707374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG16600.0295.000033
GACTGGG16250.0295.05
CGACTGG16550.0295.04
AGCGACT16650.0294.999972
ACTGGGA16250.0294.092326
CTGGGAT16200.0294.08957
AAGCGAC16800.0292.366061
TGGGATT16300.0292.285288
GGGATTA16250.0292.276929
GACTGGA351.2732926E-11252.857165
CTGGATT301.5133992E-9245.833347
ACTGGAT403.274181E-11221.256
TGGATTA353.807145E-9210.71438
ACTGCTC2400.059.00000460-64
AAACTTT303.305031E-759.00000435-39
AAACTTC303.305031E-759.00000435-39
CTTCACA150.0043663659.000004115-119
GGAGACT150.0043663659.000004135-139
AATCCCT150.0043663659.000004120-124
CCCAGGA150.0043663659.00000420-24
Produced by FastQC (version 0.11.5)