FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-67-5-8-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-67-5-8-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16536
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA470228.434929850024186No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC14038.484518626028061No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG9135.521286889211417No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8695.255200774068699No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC4282.5882922109337203No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA4012.4250120948234155No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC3952.3887276245766813No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3792.2919690372520565No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2981.8021286889211416No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC2741.6569908079342042No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2661.6086115142718915No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2611.5783744557329462No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT2491.5058055152394774No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT2091.2639090469279148No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1751.0582970488630865No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1721.0401548137397194No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCC1560.9433962264150944No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT1520.9192065795839381No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1430.8647798742138365No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT1390.8405902273826802No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1380.8345428156748911No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1180.7135945815191098No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1030.6228834059022738No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA990.5986937590711175No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT830.5019351717464925No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA820.4958877600387035No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC800.48379293662312534No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA790.4777455249153363No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA660.39912917271407833No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC580.3507498790517658No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA540.3265602322206096No Hit
CAACACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAC540.3265602322206096No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA530.3205128205128205No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTC500.3023705853894533No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC490.2963231736816643No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA490.2963231736816643No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACC480.29027576197387517No Hit
CAACACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCACTCC480.29027576197387517No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA430.26003870343492985No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACCA420.2539912917271408No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACC410.24794388001935175No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTC390.2358490566037736No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACC390.2358490566037736No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTC370.22375423318819546No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA360.21770682148040638No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC350.21165940977261732No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT350.21165940977261732No Hit
CAACACACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTCTA340.20561199806482824No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAC330.19956458635703916No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA300.18142235123367198No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT300.18142235123367198No Hit
CAACACACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTACCAC290.1753749395258829No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC290.1753749395258829No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG280.16932752781809385No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA280.16932752781809385No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTC270.1632801161103048No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTC260.15723270440251574No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT260.15723270440251574No Hit
CAACACACTGGGATTAGATACCCCTGCAGTCCTAGAGGAGCCTGTTCTAC250.15118529269472664No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCAC250.15118529269472664No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG240.14513788098693758No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACC240.14513788098693758No Hit
CAACACACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTAC230.13909046927914853No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA220.13304305757135945No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT220.13304305757135945No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCAC220.13304305757135945No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCA220.13304305757135945No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCAC210.1269956458635704No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCAC210.1269956458635704No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA200.12094823415578133No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACC190.11490082244799227No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACC190.11490082244799227No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT190.11490082244799227No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCACT180.10885341074020319No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACCA180.10885341074020319No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA180.10885341074020319No Hit
CAACACACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA180.10885341074020319No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTACCA170.10280599903241412No Hit
CAACACACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGT170.10280599903241412No Hit
CAACACACTGGGATTAGATACCCCGAGCAACCTAGAGGAGCCTGTTCTAC170.10280599903241412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.389703E-4295.08
ACACCCT108.389703E-4295.03
CACTGGG16000.0295.06
CACTGGA108.389703E-4295.06
CACCCTG108.389703E-4295.04
CTGGATT108.389703E-4295.08
CCCTGGG108.389703E-4295.06
ACCCTGG108.389703E-4295.05
TGGATTA108.389703E-4295.09
TGGGTTA108.389703E-4295.09
TGGGATA108.389703E-4295.09
ACTGGGT108.389703E-4295.07
ACTGGAT108.389703E-4295.07
CCTGGGA108.389703E-4295.07
AACTGGG157.1119957E-6294.999975
CAACTGG157.1119957E-6294.999974
ACACTGG16200.0293.179025
CTGGGAT16150.0292.260078
ACTGGGA16050.0292.242987
TGGGATT16100.0291.33549
Produced by FastQC (version 0.11.5)