FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-66-4-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-66-4-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16501
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG251515.241500515120295No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC168510.211502333191929No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA10966.64202169565481No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG8425.1027210472092595No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG8244.993636749287922No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA5843.5391794436700805No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA4212.5513605236046297No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3982.4119750318162536No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG2791.6908066177807406No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGA2641.5999030361796254No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA2571.5574813647657717No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA2391.4483970668444337No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2351.4241561117508028No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCT2351.4241561117508028No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGC1941.1756863220410882No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGC1861.127204411853827No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG1771.0726622628931581No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA1460.8847948609175201No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA1290.7817708017695897No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGA1280.7757105629961821No Hit
GCCACAACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAGC1220.739349130355736No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1210.7332888915823284No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1200.7272286528089207No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGGACCTAGAGGAGCC1120.6787467426216592No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1090.6605660263014363No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG1090.6605660263014363No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGAG1070.6484455487546209No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGC1060.6423853099812132No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1020.6181443548875826No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT1000.6060238773407672No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT950.5757226834737289No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGC950.5757226834737289No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG900.5454214896066906No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT810.49087934064602146No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGC810.49087934064602146No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAG810.49087934064602146No Hit
GCCACAACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTAGCT790.4787588630992061No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTG760.4605781467789831No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC740.44845766923216773No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCT740.44845766923216773No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTAGC730.44239743045876007No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGC680.41209623659172173No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG680.41209623659172173No Hit
GCCACAACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAG650.3939155202714987No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTG520.31513241621719895No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA520.31513241621719895No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA510.3090721774437913No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGA500.3030119386703836No Hit
GCCACAACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGCTG490.29695169989697595No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCTG490.29695169989697595No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG480.2908914611235683No Hit
GCCACAACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGCT460.2787709835767529No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAG460.2787709835767529No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT440.26665050602993756No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTG440.26665050602993756No Hit
GCCACAACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT400.2424095509363069No Hit
GCCACAACTGGGATTAGATACCCCCTCCGCGTGCTGCAGATCTAGAGGAG390.23634931216289923No Hit
GCCACAACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGCTG380.23028907338949156No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG380.23028907338949156No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA370.22422883461608387No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA360.2181685958426762No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGC360.2181685958426762No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA340.20604811829586087No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTGA330.19998787952245317No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAG320.19392764074904553No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGCTGA310.18786740197563784No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTAG290.17574692442882248No Hit
GCCACAACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTA290.17574692442882248No Hit
GCCACAACTGGGATTAGATACCCCTACCGTAGATACATGACTTTACCTAG280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGCTGAGA280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGA280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGAG280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCGGTCCTAGAGGAGCCTGTTCTAGCTG280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA280.1696866856554148No Hit
GCCACAACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG270.16362644688200714No Hit
GCCACAACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCT270.16362644688200714No Hit
GCCACAACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGC270.16362644688200714No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA270.16362644688200714No Hit
GCCACAACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAGC270.16362644688200714No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC260.15756620810859948No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA250.1515059693351918No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT240.14544573056178414No Hit
GCCACAACTGGGATTAGATACCCCTTGGTCTAGAGGAGCCTGTTCTAGCT240.14544573056178414No Hit
GCCACAACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA230.13938549178837645No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAG220.13332525301496878No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA210.12726501424156111No Hit
GCCACAACTGGGATTAGATACCCCCCCACGAGACCTAGAGGAGCCTGTTC210.12726501424156111No Hit
GCCACAACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCTA210.12726501424156111No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC200.12120477546815345No Hit
GCCACAACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGC190.11514453669474578No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA180.1090842979213381No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT180.1090842979213381No Hit
GCCACAACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA180.1090842979213381No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA170.10302405914793043No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAG170.10302405914793043No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT170.10302405914793043No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT108.389703E-4295.08
ACGGGAT108.389703E-4295.07
AACGGGA108.389703E-4295.06
CAACGGG108.389703E-4295.05
ACACTGG157.1119957E-6294.999974
CACTGGG157.1119957E-6294.999975
AACTGGG16150.0294.086676
CAACTGG16250.0293.18465
TGGGATT16200.0292.268529
GCCACAA16550.0291.435031
CTGGGAT16300.0291.380378
ACTGGGA16300.0291.380377
CCACAAC16650.0288.798772
ACAACTG16900.0282.781074
CACAACT17150.0278.65893
ACAACGG200.006678046147.54
GCCACAC352.0856773E-4126.4285661
CACACTG600.001777331173.749993
GGATTAG16200.059.00000410-14
CTTAGCC1250.059.00000430-34
Produced by FastQC (version 0.11.5)