FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-65-5-5-7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-65-5-5-7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15907
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC474229.810775130445716No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA177111.13346325517068No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT9215.789903815930093No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATT7524.72747846859873No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGG5223.2815741497453947No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4102.5774816118689885No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3802.3888853963663794No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGA2611.6407870748726974No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAG2601.634500534355944No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2491.5653485886716538No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAG2461.546488967121393No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2131.3390331300685234No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA2071.3013138869680014No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2051.2887408059344942No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGG1550.9744137800968127No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1400.8801156723455084No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGAT1300.817250267177972No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1290.8109637266612183No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1120.7040925378764066No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1010.6349405921921167No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC970.6097944301251021No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT820.5154963223737977No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT810.5092097818570441No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT810.5092097818570441No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAG790.49663670082353684No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT790.49663670082353684No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT710.44634437668950777No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC670.4211982146224933No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA620.38976551203872506No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAT620.38976551203872506No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAATAGG550.3457597284214497No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA530.3331866473879424No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTC520.3269001068711888No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT510.32061356635443516No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT510.32061356635443516No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAATAGG510.32061356635443516No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT490.30804048532092787No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT470.29546740428742063No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAA470.29546740428742063No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT450.2828943232539134No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAATAGG390.2451750801533916No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC380.23888853963663798No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT370.23260199911988433No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA360.2263154586031307No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAATAGG360.2263154586031307No Hit
CTGGATGACTGGGATTAGATACCCCCGTGCTAGAGGAGCCTGTTCTAATA340.21374237756962344No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA330.20745583705286982No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAAT320.2011692965361162No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAT310.19488275601936253No Hit
CTGGATGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC310.19488275601936253No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAATA310.19488275601936253No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAA290.1823096749858553No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT290.1823096749858553No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA280.17602313446910164No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT280.17602313446910164No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA270.16973659395234802No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA260.1634500534355944No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA240.15087697240208714No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCTAGGGCCTAGAGGAGC240.15087697240208714No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGG230.1445904318853335No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG220.13830389136857987No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAA210.13201735085182625No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAATA210.13201735085182625No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGATACCTAGAGGAGCCT210.13201735085182625No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA210.13201735085182625No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA210.13201735085182625No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA200.1257308103350726No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAATA200.1257308103350726No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC190.11944426981831899No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAAC190.11944426981831899No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG190.11944426981831899No Hit
CTGGATGACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTA170.10687118878481172No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT170.10687118878481172No Hit
CTGGATGACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA160.1005846482680581No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA160.1005846482680581No Hit
CTGGATGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACA160.1005846482680581No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAAC160.1005846482680581No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGTCTAGAGGAGC160.1005846482680581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG15600.0295.06
GACGGGA108.3868264E-4295.07
CTGGATG15800.0295.01
CGGGATT108.3868264E-4295.09
GACTGGG15550.0295.07
TGACGGG108.3868264E-4295.06
GGATGAC15850.0293.13883
TGGATGA15850.0293.13882
GATGACT15850.0291.277624
ATGACTG15800.0291.265845
ACTGGGA15750.0291.253978
CTGGGAT15800.0289.398749
ATGACGG150.002823437196.666675
ACGGGAT150.002823437196.666678
GATGACG200.006675768147.54
ACCATTT303.3028664E-759.00000445-49
CCCGGGT150.004365545759.00000420-24
CCGCCTA150.004365545759.00000420-24
CCTAAAC6200.059.00000435-39
ATAGGAG150.004365545759.00000450-54
Produced by FastQC (version 0.11.5)