FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-64-4-1-3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-64-4-1-3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16202
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC304218.775459819775335No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA220213.590914701888657No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTG13978.622392297247252No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT6974.301938032341686No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGAT6363.9254413035427724No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3452.1293667448463154No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGA3171.9565485742500925No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3051.8824836439945685No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCT3021.8639674114306874No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGAT2791.7220096284409334No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2741.691149240834465No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2671.6479446981854093No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2061.2714479693864955No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGA1971.2158992716948525No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1871.1541784964819157No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1781.0986297987902727No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATC1681.0369090235773362No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC1490.9196395506727564No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1300.8023700777681768No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGA1160.7159609924700655No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT1090.6727564498210098No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1010.6233798296506604No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC1000.6172077521293667No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT900.55548697691643No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGAT820.5061103567460807No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACA800.4937662017034934No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGAT730.4505616590544377No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC720.444389581533144No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT680.4197012714479694No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT670.41352919392667575No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAC670.41352919392667575No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC570.3518084187137391No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA570.3518084187137391No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC550.33946426367115173No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC540.33329218614985806No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT530.3271201086285644No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA520.3209480311072707No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAC510.31477595358597704No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACA470.29008764350080235No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT470.29008764350080235No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT470.29008764350080235No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC450.277743488458215No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAG440.27157141093692133No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT430.2653993334156277No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA420.25922725589433404No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT400.2468831008517467No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGA370.22836686828786568No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA370.22836686828786568No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT350.21602271324527839No Hit
ATCGGTTACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTACAG350.21602271324527839No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAC340.2098506357239847No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT340.2098506357239847No Hit
ATCGGTTACTGGGATTAGATACCCCAATAGTCCTAGAGGAGCCTGTTCTA340.2098506357239847No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACA330.20367855820269104No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTACA330.20367855820269104No Hit
ATCGGTTACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTG330.20367855820269104No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTACAG320.19750648068139737No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC310.1913344031601037No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACAG300.18516232563881002No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG300.18516232563881002No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT290.17899024811751638No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCGCTAGAGGAGCCTGTT290.17899024811751638No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGGCCTAGAGGAGCCT280.1728181705962227No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT280.1728181705962227No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTACAGAT270.16664609307492903No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT270.16664609307492903No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGTGCCCACGAGACCTAGAGGAG270.16664609307492903No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGAT260.16047401555363536No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA260.16047401555363536No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACAG250.15430193803234168No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT240.148129860511048No Hit
ATCGGTTACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTACA230.14195778298975434No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT230.14195778298975434No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAC220.13578570546846067No Hit
ATCGGTTACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC220.13578570546846067No Hit
ATCGGTTACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACA220.13578570546846067No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT210.12961362794716702No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCCCACAAGACCTAGAGGAGCC210.12961362794716702No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGAT200.12344155042587335No Hit
ATCGGTTACTGGGATTAGATACCCCTCTCCGAGCCCACGAGACTCTCCGA200.12344155042587335No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA200.12344155042587335No Hit
ATCGGTTACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA200.12344155042587335No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG190.11726947290457967No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACAG190.11726947290457967No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAC190.11726947290457967No Hit
ATCGGTTACTGGGATTAGATACCCCGAATCTAGAGGAGCCTGTTCTACAG180.111097395383286No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT180.111097395383286No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT180.111097395383286No Hit
ATCGGTTACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGA170.10492531786199236No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG170.10492531786199236No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTA170.10492531786199236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG15850.0295.06
TCGGTTC108.388291E-4295.02
TCGGTTA16000.0295.02
TCTGGGA108.388291E-4295.07
GTTCTGG108.388291E-4295.05
TTCTGGG108.388291E-4295.06
GGTTACT15900.0295.04
ATCGGTT16100.0295.01
CGGTTAC16000.0295.03
ACTGGGA15900.0294.072338
GTTACTG15900.0294.072335
TACTGGG15850.0294.06947
CTGGGAT16000.0292.234389
GGTTCTG150.0028239295196.666664
CGGTTCT150.0028239295196.666663
GGACCTG3350.059.000004105-109
TATGCCT1800.059.00000425-29
CCGCTCG351.4118996E-859.00000465-69
CGCTTAA700.059.00000490-94
CTTGGCG1900.059.000004110-114
Produced by FastQC (version 0.11.5)