FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-63-5-8-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-63-5-8-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA505331.801875511360063No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA11477.218830637547989No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT10876.841210900623072No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG6654.185285417584493No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT4752.9894895839889233No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA4502.8321480269368746No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA4122.5929888602177606No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA4062.5552268865252694No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2901.8251620618037634No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2301.447542324878847No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCA1951.2272641450059791No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1520.9566366668764554No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC1460.9188746931839638No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1380.8685253949273083No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCAC1350.8496444080810625No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1310.8244697589527346No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1310.8244697589527346No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1220.767826798413997No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1070.673421864182768No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT970.6104852413619485No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA940.5916042545157028No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA940.5916042545157028No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA790.4971993202844735No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA780.4909056580023916No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA710.446850022027818No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATA680.42796903518157214No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC650.40908804833532636No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA640.4027943860532444No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATA630.3965007237711624No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC590.3713260746428347No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAA580.36503241236075273No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA550.3461514255145069No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAG540.33985776323242495No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC540.33985776323242495No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT520.3272704386682611No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGC500.31468311410409716No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT440.2769211404116055No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC410.2580401535653597No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAT410.2580401535653597No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA400.25174649128327775No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA390.2454528290011958No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATA390.2454528290011958No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA380.23915916671911386No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC360.22657184215494997No Hit
CAACACACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTATAAGC350.22027817987286805No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA330.20769085530870413No Hit
CAACACACTGGGATTAGATACCCCACGAGCTAGAGGAGCCTGTTCTATAA320.2013971930266222No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA320.2013971930266222No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG300.1888098684624583No Hit
CAACACACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTATAAG290.18251620618037637No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA280.17622254389829442No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATA280.17622254389829442No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAT270.16992888161621247No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAAG270.16992888161621247No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTATA270.16992888161621247No Hit
CAACACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTATA270.16992888161621247No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA250.15734155705204858No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT250.15734155705204858No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT250.15734155705204858No Hit
CAACACACTGGGATTAGATACCCCGACGTGCTAGAGGAGCCTGTTCTATA250.15734155705204858No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAT230.1447542324878847No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGGGACCTAGAGGAGCCTG230.1447542324878847No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA230.1447542324878847No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCA220.13846057020580274No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG210.13216690792372082No Hit
CAACACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG210.13216690792372082No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA210.13216690792372082No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG210.13216690792372082No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATA200.12587324564163888No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCA200.12587324564163888No Hit
CAACACACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAAG190.11957958335955693No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAG190.11957958335955693No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATAAG190.11957958335955693No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA180.11328592107747498No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACCTTGATA180.11328592107747498No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA180.11328592107747498No Hit
CAACACACTGGGATTAGATACCCCGATCCCACGAGACCTAGAGGAGCCTG170.10699225879539304No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA170.10699225879539304No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATAAG170.10699225879539304No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCA170.10699225879539304No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT170.10699225879539304No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA160.1006985965133111No Hit
CAACACACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTAT160.1006985965133111No Hit
CAACACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG160.1006985965133111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG157.1066843E-6295.06
ACACGGG108.3865755E-4295.05
CACGCTG157.1066843E-6295.04
CACTGGG15150.0295.06
CTGGATT157.1066843E-6295.08
CGGGATT108.3865755E-4295.08
ACGGGAT108.3865755E-4295.07
ACACTGG15450.0294.045325
ACTGGGA15200.0294.02967
CTGGGAT15450.0293.090648
TGGGATT15500.0291.193579
CACACTG15700.0290.302554
ACACACT16550.0276.283973
CACTGGA251.8246465E-7236.06
ACTGGAT251.8246465E-7236.07
AACACAC19500.0235.999982
CAACACA20200.0228.551991
ACACGCT202.2400589E-5221.253
ACGCTGG202.2400589E-5221.255
TGGATTA202.2400589E-5221.259
Produced by FastQC (version 0.11.5)