FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-62-4-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-62-4-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15927
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA268016.826772147924906No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGC170210.686256043197087No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC10236.423055189301187No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAAT8255.179883217178376No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAA6183.880203428140893No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5453.4218622464996544No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC5323.340239844289571No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT4392.756325736171281No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATC3942.4737866515979157No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCG3792.379606956740127No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3252.0405600552520875No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAAT2691.6889558611163435No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2461.544546995667734No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAA2451.5382683493438816No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGGA2311.4503673008099454No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1921.205500094179695No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT1841.1552709235888743No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGG1621.0171407044641174No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGA1540.966911533873297No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGG1280.8036667294531299No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG1170.7346016198907516No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA1120.7032083882714887No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAAT970.6090286934137No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAA950.5964714007659948No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG910.5713568154705846No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAAT870.5462422301751743No Hit
CTGGATGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA830.5211276448797639No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT790.4960130595843536No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA770.4834557669366485No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAAT770.4834557669366485No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAAT740.4646198279650907No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGA740.4646198279650907No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG730.4583411816412381No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT700.4395052426696804No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT690.43322659634582783No Hit
CTGGATGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGG680.4269479500219752No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT670.4206693036981227No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT650.4081120110504175No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC610.3829974257550072No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT600.37671877943115467No Hit
CTGGATGACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA590.37044013310730206No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG570.3578828404595969No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT570.3578828404595969No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTTAGAGGAGCCTGTTCT530.3327682551641866No Hit
CTGGATGACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGA520.32648960884033407No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCGACGACTAGAGGAGCCTGT510.32021096251648146No Hit
CTGGATGACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTA510.32021096251648146No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT490.3076536698687763No Hit
CTGGATGACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAGGAA480.30137502354492374No Hit
CTGGATGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGG480.30137502354492374No Hit
CTGGATGACTGGGATTAGATACCCCAACTTAGAGGAGCCTGTTCTAGGAA450.28253908457336596No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC440.2762604382495134No Hit
CTGGATGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGGAA400.25114585295410313No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT340.2134739750109876No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG320.20091668236328247No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC320.20091668236328247No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGA310.1946380360394299No Hit
CTGGATGACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAGG300.18835938971557734No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA300.18835938971557734No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGA300.18835938971557734No Hit
CTGGATGACTGGGATTAGATACCCCAGGAGACCTAGAGGAGCCTGTTCTA290.18208074339172475No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT290.18208074339172475No Hit
CTGGATGACTGGGATTAGATACCCCGTATAAGAGGAGCCTGTTCTAGGAA290.18208074339172475No Hit
CTGGATGACTGGGATTAGATACCCCAAGAATCTGCTAGAGGAGCCTGTTC280.17580209706787217No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGGA270.1695234507440196No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG270.1695234507440196No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC270.1695234507440196No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAA260.16324480442016703No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGGA240.15068751177246187No Hit
CTGGATGACTGGGATTAGATACCCTAAGAGGAGCCTGTTCTAGGAATCGC230.14440886544860929No Hit
CTGGATGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA200.12557292647705157No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG190.11929428015319897No Hit
CTGGATGACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTT170.1067369875054938No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT160.10045834118164124No Hit
CTGGATGACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG160.10045834118164124No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC160.10045834118164124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG108.3868264E-4295.06
GATGGGA108.3868264E-4295.07
ATGGGAT108.3868264E-4295.08
GGATGAT108.3868264E-4295.03
CGGGATT108.3868264E-4295.09
GGATGAC15750.0294.06353
ATGACTG15650.0294.057535
CTGGGAT15600.0294.05459
ACTGGGA15550.0294.051458
TGGATGA15900.0293.144652
TGACTGG15700.0293.121036
GACTGGG15650.0293.115027
CTGGATG16050.0292.242981
GATGACT15800.0291.265844
ATGACGG150.002823437196.666675
GACGGGA150.002823437196.666677
GATGATG150.002823437196.666674
ACGGGAT150.002823437196.666678
TGACGGG200.006675768147.56
CGACACC150.004365545759.000004185-189
Produced by FastQC (version 0.11.5)