FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-61-5-2-6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-61-5-2-6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15685
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA520433.17819572840293No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA11567.370098820529168No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA8905.674211029646159No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTC6163.9273190946764425No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA5863.7360535543512907No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA4602.932738284985655No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGA4332.760599298693019No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA2721.7341408989480396No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2361.504622250557858No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2271.4472425884603124No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAGG1951.2432260121134842No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGGA1911.2177239400701307No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1671.0647115078100096No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATT1360.8670704494740198No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1290.822441823398151No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1270.8096907873764744No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1160.7395600892572521No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAGG1150.7331845712464138No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA1120.7140580172138986No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1080.6885559451705451No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAGG1020.6503028371055148No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA940.5992986930188078No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA930.5929231750079694No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA880.5610455849537774No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA860.5482945489321007No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGAT860.5482945489321007No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG820.5227924768887472No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT790.5036659228562321No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAT780.4972904048453937No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC780.4972904048453937No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA750.4781638508128785No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAATAGGA580.36978004462862607No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA560.3570290086069493No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA520.3315269365635958No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAT520.3315269365635958No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAATAGG500.318775900541919No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAT450.2868983104877271No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT450.2868983104877271No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA440.2805227924768887No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAATA430.27414727446605036No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC410.2613962384443736No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGGA400.2550207204335352No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA400.2550207204335352No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT380.24226968441185848No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC320.20401657634682818No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAATAG320.20401657634682818No Hit
GCCACAACTGGGATTAGATACCCCGAGGACCTAGAGGAGCCTGTTCTAAT310.19764105833598983No Hit
GCCACAACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAAT310.19764105833598983No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG290.18489002231431304No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAT280.17851450430347465No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAATAGGA280.17851450430347465No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT270.17213898629263627No Hit
GCCACAACTGGGATTAGATACCCCTAGCCCACGAGACCTAGAGGAGCCTG260.1657634682817979No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA250.1593879502709595No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAA240.15301243226012112No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA240.15301243226012112No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAATA240.15301243226012112No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC240.15301243226012112No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAATAG230.14663691424928277No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT220.14026139623844436No Hit
GCCACAACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAATAGG220.14026139623844436No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAA220.14026139623844436No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGA220.14026139623844436No Hit
GCCACAACTGGGATTAGATACCCCGAGCAACCTAGAGGAGCCTGTTCTAA210.133885878227606No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA210.133885878227606No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA210.133885878227606No Hit
GCCACAACTGGGATTAGATACCCCGGCCTAGAGGAGCCTGTTCTAATAGG190.12113484220592924No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAAT190.12113484220592924No Hit
GCCACAACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAATA190.12113484220592924No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAATA190.12113484220592924No Hit
GCCACAACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTAATAGGA190.12113484220592924No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG180.11475932419509084No Hit
GCCACAACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAATAGGA170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGC170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAATAG170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAT170.10838380618425247No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAA160.10200828817341409No Hit
GCCACAACTGGGATTAGATACCCCCGAGATCTAGAGGAGCCTGTTCTAAT160.10200828817341409No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAT160.10200828817341409No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA160.10200828817341409No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT160.10200828817341409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG108.3855615E-4295.05
CTGGATT255.0749804E-10295.08
TGGATTA255.0749804E-10295.09
GCCACAC108.3855615E-4295.01
GCCACAA15550.0295.01
ACTGGAT255.0749804E-10295.07
CAACTGG15350.0294.999975
AACTGGG15100.0294.023166
CTGGGAT15000.0293.033338
ACTGGGA15100.0292.069527
TGGGATT14950.0292.040139
CCACAAC15850.0287.55522
CACAACT15950.0285.752353
ACAACTG16200.0280.43214
AACTGGA403.274181E-11221.256
ACACTGG150.0028230126196.666674
TGGGATA150.0028230126196.666679
AACGCTT452.5465852E-1159.00000490-94
AATCTTG452.5465852E-1159.00000440-44
TAAACTC452.5465852E-1159.00000435-39
Produced by FastQC (version 0.11.5)