FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-60-5-2-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-60-5-2-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA444128.77786417833074No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG9866.389320891653706No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT8205.3136340072576465No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA7795.047952306894764No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG5363.4733022291342666No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA4522.928978745463971No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3272.1189735614307934No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG3192.0671332296526694No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT3132.0282529808190772No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT2871.8597719025401762No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2191.4191290824261276No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTG2101.3608087091757388No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1991.2895282529808192No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGC1941.257128045619492No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1611.043286677034733No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGA1420.92016588906169No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1360.8812856402280975No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1340.8683255572835666No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC1270.8229652669777087No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG1220.7905650596163815No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1130.7322446863659927No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGG1080.6998444790046656No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGT1020.6609642301710731No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA1010.6544841886988076No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1010.6544841886988076No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGT970.628564022809746No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA950.6156039398652151No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA800.5184033177812338No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA790.5119232763089684No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA790.5119232763089684No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA730.47304302747537585No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGACG690.44712286158631415No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGA670.4341627786417833No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC650.42120269569725244No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA620.4017625712804562No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA580.3758424053913945No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT580.3758424053913945No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG570.3693623639191291No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG550.35640228097459825No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA550.35640228097459825No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA510.33048211508553654No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGA500.32400207361327116No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT480.3110419906687403No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGAC470.3045619491964749No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT440.2851218247796786No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGT390.2527216174183515No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGAC370.23976153447382065No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTG370.23976153447382065No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGA360.23328149300155523No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG360.23328149300155523No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC350.2268014515292898No Hit
GCCACAACTGGGATTAGATACCCCTAGTAGTCCTAGAGGAGCCTGTTCTA340.22032141005702438No Hit
GCCACAACTGGGATTAGATACCCCCACAGAGAGCTAGAGGAGCCTGTTCT330.21384136858475894No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA320.20736132711249353No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA310.2008812856402281No Hit
GCCACAACTGGGATTAGATACCCCTGGTGACGACTAGAGGAGCCTGTTCT300.19440124416796267No Hit
GCCACAACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGACG300.19440124416796267No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT290.18792120269569726No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA290.18792120269569726No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT280.18144116122343182No Hit
GCCACAACTGGGATTAGATACCCCCGACAACTAGAGGAGCCTGTTCTAGA280.18144116122343182No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA270.1749611197511664No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTG270.1749611197511664No Hit
GCCACAACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTAG240.15552099533437014No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGA230.14904095386210472No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT230.14904095386210472No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA220.1425609123898393No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGA220.1425609123898393No Hit
GCCACAACTGGGATTAGATACCCCTGAAGACCTAGAGGAGCCTGTTCTAG220.1425609123898393No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGACG220.1425609123898393No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG210.13608087091757387No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGACG200.12960082944530846No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG190.12312078797304302No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGACG190.12312078797304302No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTG190.12312078797304302No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGACG190.12312078797304302No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTG180.11664074650077762No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG180.11664074650077762No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCT160.10368066355624676No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG160.10368066355624676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG15150.0295.000036
CAACTGG15250.0295.05
ACTGGGA15200.0293.05927
CTGGGAT15150.0293.052838
TGGGATT15100.0293.046369
GCCACAA15500.0291.193541
CCACAAC15850.0284.76342
ACAACTG15950.0282.053284
CACAACT16050.0281.214973
AACTGGA150.0028225745196.666676
CCCCGAC452.5465852E-1159.00000420-24
AAACCGC501.8189894E-1259.060-64
TTCACCA201.836616E-459.065-69
AACCGCT550.059.060-64
CTGAGCA501.8189894E-1259.050-54
TAAACCC351.4106263E-859.035-39
TAAATCT351.4106263E-859.035-39
CCAGCTT1400.059.085-89
ACCATTT501.8189894E-1259.045-49
CCCGGTA201.836616E-459.020-24
Produced by FastQC (version 0.11.5)