FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-6-4-4-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-6-4-4-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11230
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG195517.40872662511131No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT133111.852181656277827No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA10869.670525378450579No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCAC4964.416740872662511No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3503.1166518254674975No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATA3453.0721282279608193No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCAC3092.751558325912734No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCACT2472.19946571682992No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGCA2342.0837043633125556No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACTG1991.772039180765806No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGCA1981.7631344612644702No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATA1921.7097061442564558No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1511.344612644701692No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1441.2822796081923418No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1241.1041852181656278No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1181.0507569011576134No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT920.8192341941228851No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG880.7836153161175422No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATAA800.7123775601068566No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATAA710.6322350845948354No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATA710.6322350845948354No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG580.5164737310774711No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT570.5075690115761353No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAGCA550.4897595725734639No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCACT520.4630454140694568No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT500.44523597506678536No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT490.43633125556544966No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATA490.43633125556544966No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG480.42742653606411396No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAAGC480.42742653606411396No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCAC440.3918076580587711No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTATAAGCA440.3918076580587711No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG410.365093499554764No Hit
GGTACACTGGGATTAGATACCCCCGACTAGAGGAGCCTGTTCTATAAGCA410.365093499554764No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCAC410.365093499554764No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG400.3561887800534283No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTATA400.3561887800534283No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATAA380.3383793410507569No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATAAGC380.3383793410507569No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGCA380.3383793410507569No Hit
GGTACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTATA380.3383793410507569No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG370.3294746215494212No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTATAAG350.3116651825467498No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAAG350.3116651825467498No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG330.2938557435440784No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT320.28495102404274264No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG310.27604630454140694No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATAAGC270.2404274265360641No Hit
GGTACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTATAAGC270.2404274265360641No Hit
GGTACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT270.2404274265360641No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT270.2404274265360641No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCAC270.2404274265360641No Hit
GGTACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTATAA260.2315227070347284No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATAA250.22261798753339268No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATAAG250.22261798753339268No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTATAA240.21371326803205698No Hit
GGTACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTATAAG230.20480854853072128No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTATA230.20480854853072128No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG230.20480854853072128No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAT220.19590382902938555No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATA220.19590382902938555No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG220.19590382902938555No Hit
GGTACACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAAGC220.19590382902938555No Hit
GGTACACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTATAAGC210.18699910952804988No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT210.18699910952804988No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAAGC210.18699910952804988No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATAA210.18699910952804988No Hit
GGTACACTGGGATTAGATACCCCTGCGTGCCGCGACTAGAGGAGCCTGTT210.18699910952804988No Hit
GGTACACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTCTAT200.17809439002671415No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATAAGC190.16918967052537845No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGCAC190.16918967052537845No Hit
GGTACACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATAA190.16918967052537845No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCAC180.16028495102404272No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATAA180.16028495102404272No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT180.16028495102404272No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCACT180.16028495102404272No Hit
GGTACACTGGGATTAGATACCCCGCTGCTGGAGACTAGAGGAGCCTGTTC180.16028495102404272No Hit
GGTACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAGCA170.15138023152270705No Hit
GGTACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTATAAGC160.14247551202137132No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTATAAGCAC150.13357079252003562No Hit
GGTACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTATAA150.13357079252003562No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA150.13357079252003562No Hit
GGTACACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC140.1246660730186999No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCAC140.1246660730186999No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATAAGCA140.1246660730186999No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG130.1157613535173642No Hit
GGTACACTGGGATTAGATACCCCAAGATCGAAAGAGCGCTAGAGGAGCCT130.1157613535173642No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTATA130.1157613535173642No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG130.1157613535173642No Hit
GGTACACTGGGATTAGATACCCCATTCTCGTTCTAGAGGAGCCTGTTCTA130.1157613535173642No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT120.10685663401602849No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT120.10685663401602849No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAGC120.10685663401602849No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG120.10685663401602849No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATAAGCA120.10685663401602849No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.353645E-4295.06
CTGGGAT10850.0295.07
CACTGGG10950.0295.05
CACTGGA108.353645E-4295.05
TACATGG108.353645E-4295.03
ACTGGGA10900.0295.06
GGTACAC11100.0295.01
ACACTGG11150.0292.354254
TGGGATT10950.0292.305948
GGGATTA10900.0292.293589
GTACACT11350.0288.50222
TACACTG11350.0288.50223
ACATGGG150.0028122955196.666674
GTACATG150.0028122955196.666672
CATGGGA150.0028122955196.666675
GGTACAT150.0028122955196.666671
TGATATC452.5465852E-1159.00000460-64
CCCGTCT452.5465852E-1159.00000420-24
ATGCCTA452.5465852E-1159.00000425-29
TGCTTAG2750.059.00000425-29
Produced by FastQC (version 0.11.5)