FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-59-4-5-7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-59-4-5-7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15279
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC254616.663394201191178No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA212013.875253616074351No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCC159010.406440212055763No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTC6424.201845670528176No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5583.6520714706459847No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT4352.8470449636756334No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3852.51979841612671No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTT3812.4936186923227956No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGC2141.4006152235093918No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTC2111.3809804306564566No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTT2011.3155311211466718No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1921.2566267425878657No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTC1851.2108122259310163No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1831.1977223640290595No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1831.1977223640290595No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCG1761.1519078473722102No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAC1601.0471889521565547No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1510.9882845735977485No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1450.9490149878918778No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1260.8246612998232868No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1150.7526670593625238No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1080.7068525427056745No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT960.6283133712939328No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT920.602133647490019No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACT900.589043785588062No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACT900.589043785588062No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT860.5628640617841482No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAAC790.517049545127299No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA720.4712350284704497No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC660.4319654427645789No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC660.4319654427645789No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA620.40578571896066495No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACT590.38615092610772955No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT590.38615092610772955No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAC570.3730610642057726No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA570.3730610642057726No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA570.3730610642057726No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC560.36651613325479415No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT550.3599712023038157No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAACT530.34688134040185875No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA530.34688134040185875No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTC510.3337914784999019No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT510.3337914784999019No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTC480.3141566856469664No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTC460.3010668237450095No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT460.3010668237450095No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC450.294521892794031No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTT440.28797696184305255No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA420.2748870999410956No Hit
CTGGATGACTGGGATTAGATACCCCCGCACCCTAGAGGAGCCTGTTCTAA370.24216244518620328No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA370.24216244518620328No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGCCCTAGAGGAGCCTGTTCT360.23561751423522484No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT350.22907258328424634No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTC350.22907258328424634No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAA330.21598272138228944No Hit
CTGGATGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAC320.20943779043131094No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT320.20943779043131094No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC320.20943779043131094No Hit
CTGGATGACTGGGATTAGATACCCCTGTTATTTCCTAGAGGAGCCTGTTC310.20289285948033248No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT300.19634792852935404No Hit
CTGGATGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAA290.18980299757837554No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT280.18325806662739708No Hit
CTGGATGACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA270.17671313567641864No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT270.17671313567641864No Hit
CTGGATGACTGGGATTAGATACCCCCGCGGACCTAGAGGAGCCTGTTCTA260.17016820472544014No Hit
CTGGATGACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTC230.15053341187250474No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTT230.15053341187250474No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.14398848092152627No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT220.14398848092152627No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA220.14398848092152627No Hit
CTGGATGACTGGGATTAGATACCCCGCTGCTAGAGGAGCCTGTTCTAACT220.14398848092152627No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTC210.1374435499705478No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT210.1374435499705478No Hit
CTGGATGACTGGGATTAGATACCCCCAAACCTAGAGGAGCCTGTTCTAAC200.13089861901956934No Hit
CTGGATGACTGGGATTAGATACCCCCCTCCGACAATCGCGATTCGAACTG190.12435368806859087No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA180.11780875711761242No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAA180.11780875711761242No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAACTTCG180.11780875711761242No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG170.11126382616663394No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA160.10471889521565547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC15200.0295.03
TGACTGG15100.0295.06
ATGACGG108.3834527E-4295.05
GACTGGG15050.0295.07
TGACGGG108.3834527E-4295.06
CTGGATG15300.0294.035981
TGGATGA15250.0294.03282
CTGGGAT15100.0294.023169
ACTGGGA15100.0294.023168
ATGACTG15200.0293.05925
GATGACT15200.0293.05924
GATGACG150.0028223048196.666664
GCCTCAG750.059.000004115-119
CCAAAGG750.059.000004100-104
CCAAAAA750.00429821559.000004295
TATGCCT900.059.00000425-29
CGCCAGC750.059.00000485-89
GCCAGGG750.059.00000470-74
GCCAGCT750.059.00000485-89
CCGTAAA750.059.00000435-39
Produced by FastQC (version 0.11.5)