FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-58-4-1-3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-58-4-1-3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15436
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC275817.867323140710027No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA195412.658719875615446No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATT13568.784659238144597No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGG6654.308110909562063No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT5503.5630992485099764No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4673.025395180098471No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAG4062.6302150816273646No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGAT3932.545996372117129No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGA2721.762114537444934No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGG2591.677895827934698No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2311.4965016843741903No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2231.444674786214045No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2011.3021508162736462No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGG1811.1725835708732832No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1781.1531484840632287No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1510.978232702772739No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1470.9523192536926665No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1430.9264058046125938No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1390.9004923555325215No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1300.8421870951023581No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAT1210.7838818346721949No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1100.7126198497019953No Hit
ATCGGTTACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAA1060.6867064006219228No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAATAG1000.647836227001814No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAG990.6413578647317958No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT830.5377040684115055No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT800.5182689816014512No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA710.4599637211712879No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAATA710.4599637211712879No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGA690.44700699663125165No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAATA690.44700699663125165No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA660.42757190982119725No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAA640.4146151852811609No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC640.4146151852811609No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAT610.3951800984711065No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAATA600.38870173620108833No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT580.3757450116610521No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAATAGG570.36926664939103393No Hit
ATCGGTTACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTAAT550.35630992485099766No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA530.3433532003109614No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAATAGG530.3433532003109614No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC490.3174397512308888No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAA480.31096138896087067No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA480.31096138896087067No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT480.31096138896087067No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT450.29152630215081626No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCCAGAGGAGCCTGTTCTA450.29152630215081626No Hit
ATCGGTTACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAAT450.29152630215081626No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAATA430.27856957761078No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAG400.2591344908007256No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA400.2591344908007256No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT380.2461777662606893No Hit
ATCGGTTACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA380.2461777662606893No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA370.23969940399067116No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAATAGG360.233221041720653No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT360.233221041720653No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC360.233221041720653No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT350.22674267945063487No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT320.20730759264058046No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCGACCTAGAGGAGCCTGTTCT320.20730759264058046No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAATA320.20730759264058046No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT320.20730759264058046No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT310.20082923037056233No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAT310.20082923037056233No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGT290.18787250583052606No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT290.18787250583052606No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT270.17491578129048976No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGCGCAGCTGTTGGAGACT270.17491578129048976No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAATA250.1619590567504535No Hit
ATCGGTTACTGGGATTAGATACCCCTAGTAGTCCTAGAGGAGCCTGTTCT230.14900233221041723No Hit
ATCGGTTACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTA220.14252396994039906No Hit
ATCGGTTACTGGGATTAGATACCCCTGCGTGCCGCGACTAGAGGAGCCTG220.14252396994039906No Hit
ATCGGTTACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTT220.14252396994039906No Hit
ATCGGTTACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAATAG210.13604560767038093No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAATA210.13604560767038093No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCT200.1295672454003628No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT190.12308888313034465No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCC190.12308888313034465No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGG180.1166105208603265No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA180.1166105208603265No Hit
ATCGGTTACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTAATAGG170.11013215859030838No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA170.11013215859030838No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC170.11013215859030838No Hit
ATCGGTTACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC160.10365379632029023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG15050.0294.97736
GGTTACT15250.0294.977264
CGGTTAC15250.0294.977263
ATCGGTT15400.0294.019561
TCGGTTA15300.0294.01332
GTTACTG15250.0294.010135
TACTGGG14950.0293.990727
ACTGGGA14900.0293.98748
CTGGGAT14950.0293.004189
TACTGAG202.2395086E-5221.232967
CTGGATT150.0028232252196.651549
CTGAGAT255.452941E-5176.986369
TTACTGA255.452941E-5176.986366
ACTGAGA301.1277007E-4147.488658
TACTGGA200.006675271147.488637
CCCGTCT850.059.18762620-24
CCCCGTC850.059.18762620-24
ACCCCGT850.059.18762620-24
CCCCACT5300.059.18762620-24
CCTGCTA150.004310439359.18762220-24
Produced by FastQC (version 0.11.5)