FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-57-4-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-57-4-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15271
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC268717.595442341693406No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA13558.873027306659683No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGC12518.191997904524918No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGT7454.878527928753847No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA7404.84578613057429No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT4633.0318905114268877No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACG3652.3901512671075897No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGT3372.206797197302076No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACG3282.147861960578875No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2601.7025735053369133No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2251.473380918080021No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2091.3686071639054416No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2031.3293170060899746No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGG2001.3096719271822408No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1891.2376399711872175No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1841.2048981730076616No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1791.1721563748281056No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTG1611.0542859013817039No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACG1601.0477375417457926No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1260.8250933141248118No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAG1230.8054482352170782No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGA1140.7465129984938773No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGAC1090.7137712003143213No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACG1040.6810294021347653No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1030.674481042498854No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1010.6613843232270317No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1000.6548359635911204No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA950.6220941654115644No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGT930.608997446139742No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT780.5107720516010739No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG760.49767533232925154No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG720.47148189378560673No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTA720.47148189378560673No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAC670.43874009560605065No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC670.43874009560605065No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC660.4321917359701395No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA660.4321917359701395No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG610.39944993779058346No Hit
CTGGATGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGAC600.39290157815467225No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT590.38635321851876103No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACG520.34051470106738263No Hit
CTGGATGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAGA500.3274179817955602No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG500.3274179817955602No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT460.3012245432519154No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGAC460.3012245432519154No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGT460.3012245432519154No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGAC450.2946761836160042No Hit
CTGGATGACTGGGATTAGATACCCCTAGGATTGTTGGCAAGCTAGAGGAG450.2946761836160042No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAG430.2815794643441818No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT390.25538602580053693No Hit
CTGGATGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAG390.25538602580053693No Hit
CTGGATGACTGGGATTAGATACCCCCGTGATGCTAGAGGAGCCTGTTCTA360.23574094689280337No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA360.23574094689280337No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAG360.23574094689280337No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCTAGAGGAGCCTGTTCTAGA350.22919258725689215No Hit
CTGGATGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG340.22264422762098093No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGT320.20954750834915856No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC310.20299914871324734No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCCAGAGGAGCCTGTTCTA300.19645078907733612No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT300.19645078907733612No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGCGCAGCTGTTGGAGACT300.19645078907733612No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG300.19645078907733612No Hit
CTGGATGACTGGGATTAGATACCCCTCTTATCACATCTGACGCTAGAGGA290.18990242944142494No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTC280.18335406980551372No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT280.18335406980551372No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT280.18335406980551372No Hit
CTGGATGACTGGGATTAGATACCCCCCTCGTCTACGGGAGTAGAGAGCCT270.1768057101696025No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGT260.17025735053369132No Hit
CTGGATGACTGGGATTAGATACCCCGGATAGTCCCTAGAGGAGCCTGTTC250.1637089908977801No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGA240.1571606312618689No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCAGACCCTAGAGGAGCC240.1571606312618689No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.14406391199004648No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT220.14406391199004648No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC220.14406391199004648No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGAC210.1375155523541353No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT200.13096719271822407No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT190.12441883308231289No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC190.12441883308231289No Hit
CTGGATGACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTA190.12441883308231289No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT180.11787047344640168No Hit
CTGGATGACTGGGATTAGATACCCCGTTGGCCGAGATGTCTAGAGGAGCC170.11132211381049047No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC170.11132211381049047No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA160.10477375417457928No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT160.10477375417457928No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGT160.10477375417457928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGG14950.0295.07
CTGGATG15250.0294.03281
TGGATGA15250.0294.03282
GGATGAC15200.0294.02963
ACTGGGA15000.0294.016668
CTGGGAT14950.0294.013379
TGACTGG15150.0293.05286
GATGACT15300.0292.107854
ATGACTG15450.0288.317145
CTGGATT150.0028223048196.666669
ACTGGAT200.006673096147.58
TAGCCGT900.059.00000430-34
CCGTAAA900.059.00000435-39
ACCCCGG900.059.00000420-24
CCACAGC3000.059.00000485-89
ACCCCCA452.5465852E-1159.00000420-24
CTAGCCG900.059.00000430-34
TTGGCGG1500.059.000004110-114
GCCGTAA900.059.00000435-39
GACCTGG3100.059.000004105-109
Produced by FastQC (version 0.11.5)